摘要
目的探讨复方氨基酸联用维生素B6疗法对创伤凝血病大鼠肝脏凝血因子表达的作用。方法选择雄性SD鼠30只，按随机数字表法分为正常组、假手术组、新疗法组及对照组，而新疗法组和对照组又分为伤后4h，6h，12h及24h四个时间点处理，每组3只。采用Feeney’s自由落体硬膜外撞击法加大腿骨折与放血制作大鼠创伤性凝血病模型，正常组行颈静脉插管输注复方氨基酸与维生素B6，而对照组输注等量的生理盐水。按上述时间点取肝脏组织，Trizol法提取总RNA，实时荧光定量PCR法检测肝脏凝血因子（FⅡ，FⅦ，FⅨ，FⅩ，FⅪ，FⅫ，FⅧA亚基）mRNA表达水平变化。结果与正常组比较，除FⅥ与FⅧA亚基外，新疗法组和对照组的肝脏凝血因子基因mRNA表达上调，但随着造模时间延长，其表达水平逐渐下降；新疗法组与对照组比较，肝脏凝血因子基因mRNA表达水平更高，差异具有统计学意义（P〈0．05）。结论复方氨基酸联用维生素B6疗法能够显著提高肝脏凝血因子基因mRNA表达水平，促进凝血因子在肝脏中的合成，改善创伤凝血病大鼠的凝血功能。
Objective To investigate the effect of new therapy of 20 AA compound amino acid injection plus high-dose vitamin B6 on the gene expression of coagulation factors in liver of rat with trauma induced coagulophathy （TIC） model. Methods Thirty male SD rats were randomly （random number） divided into normal group, sham group, control group and new therapy group, and then control group and new therapy group were divided into four sub-groups （4 h, 6 h, 12 h and 24 h after injury, n =3 in each group）. The multiple trauma model of rat was established by using Feeney ＂s free fall epidural impact method, plus bilateral femoral fractures and bleeding. According to above intervals, the total RNA was isolated from liver tissue by Trizol method, the mRNA expression level of coagulation factor genes （ F Ⅱ , F Ⅶ, FⅨ, F Ⅹ, F Ⅺ, F Ⅻ, F X Ⅲ A subunit） were determined by real-time PCR method. Results Compared with normal group, except for F Ⅺ and X Ⅲ A subunit, the expressions of coagulation factor genes in control group and new therapy group were up-regulated significantly from 4 h after injury, but the expressions were down-regulated with time prolonged. Compared with control group, the expression was higher in the new therapy group （P 〈 0. 05 ）. Conclusions The new therapy can improve the coagulation function by enhancing the expression of the coagulation factor genes in the liver.
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2016年第5期586-591,共6页
Chinese Journal of Emergency Medicine
基金
国家重点实验室开放课题（SKLKF201312）
江苏省卫生厅重大课题（Z201013）
常州市应用基础研究课题（CJ20140001）
关键词
创伤性凝血病
20AA复方氨基酸
维生素B6
凝血因子
基因表达
Trauma-induced coagulopatby
20 AA compound amino acid injection
Vitamin B6
Coagulation factor
Gene expression
