摘要
采用Hi-Plex钙型强阳离子交换柱,以超纯水为流动相、示差折光仪为检测器,在进样量15μL、流速0.4mL/min、柱温80℃的条件下建立了高效液相色谱法同时测定烟草中的果糖、葡萄糖、麦芽糖、蔗糖和甘油、丙二醇、山梨醇的方法。结果表明,该方法检测限、回收率、相对标准偏差分别在1.5~2.0 mg/L、97%~108%、0.89%~1.39%。同时,利用该方法对13种国内外品牌卷烟进行分析,结果发现所有卷烟样品中均检出了丙二醇和果糖、葡萄糖、麦芽糖、蔗糖,而仅在中国产卷烟1和C牌号进口卷烟中检出丙三醇的存在,表明大部分卷烟基本上不用甘油作保润剂。同时发现进口卷烟不同价位间所使用保润剂比例不同,其配方保润剂的设计思路可能充分考虑了烟叶类型的差异,值得深入探讨。
High performance liquid hromatography （HPLC） was de veloped to detect fructose, glucose, maltose, sucrose, glycerol, pro- pylene glycol and sorbitol in tobacco. Samples were separated in Hi- Plex Ca cation ion exchange column with ultra pure water as mobile phase, refractive index detector as detector, and injection volume 15 μL, velocity of 0.4 mL/min, column temperature 80℃. The resuits show that the recoveries of sugars and polyols ranged from 97% to 108% with RSD of 0. 89% -1. 39% in the limit of detection of 1.5--2.0 mg/L. Meanwhile. Thirteen domestic and imported ciga rette brands were analyzed by using this method. Results indicate that the propylene glycol, fructose, glucose, maltose and sucrose were detected in all cigarettes, while glycerol detected only in doraes tie cigarette No. 1 and imported brand series C, this means that most cigarettes did not use glycerol as humectant. And it was found that imported brand series with two different prices use different propor- tions of glycerol and propylene glycol. Thus, the humectant formula of imported cigarette may give full consideration in tobacco leaf type, and it maybe worth exploring.
出处
《食品与机械》
CSCD
北大核心
2016年第3期73-76,共4页
Food and Machinery
基金
江西中烟工业有限责任公司技术创新项目(编号:赣烟工科技2014-03)
关键词
高效液相色谱法
糖
多元醇
卷烟
品牌
high performance liquid chromatography
sugar
humectant
cigarette
brand
