摘要
目的查明引起内蒙古自治区呼伦贝尔市鼠类感染肾综合征出血热（HFRS）的病毒基因型及亚型。方法2014年春季在呼伦贝尔市莫力达瓦旗HFRS疫区捕获啮齿类宿主动物，对捕获的宿主动物进行分类鉴定。取90只鉴定后的鼠进行无菌解剖取肺组织．提取RNA．用实时荧光定量反转录PCR（Real．TimeRT—PCR）扩增汉滩型病毒（HTNV）、汉城型病毒（SEOV）及普拉马病毒（PUUV），对扩增结果为阳性标本以反转录PCR（RT-PCR）法扩增部分S基因片段，将目的片段测序且与已知病毒序列进行系统发生分析并分型。结果呼伦贝尔市HFRS疫区是以姬鼠型为主的混合疫区，捕获鼠类共10种362只，鼠类平均密度为13．92％（362／2600）。野外以黑线姬鼠为优势鼠种（占43．21％，70／162），其次为大林姬鼠（占34．57％，56／162）；居民区以褐家鼠为优势鼠种（占40．50％，81／200），其次为黑线姬鼠（占31．50％，63／200）。在90份鼠肺组织中，23份鼠肺样品检测到汉坦病毒（HV），且均为HTNV。对10株部分S片段核苷酸产物进行序列测定，均为HTNV的H6亚型。结论引起内蒙古自治区呼伦贝尔市鼠类感染HFRS的病毒基因型为HTNV，其S基因亚型为H6。
Objective To investigate the genotypes and subtypes of hemorrhagic fever with renal syndrome （HFRS） in Hulunbeier, Inner Mongolia Autonomous Region. Methods In the spring of 2014, rats were collected from the main endemic areas in Morin Dawa Qi in Hulunbeier. RNA was extracted from 90 rat lungs, identified and classified under aseptic conditions. Hantao：n virus （HTNV）, Seoul virus （SEOV） and Puumda virus （PUUV） of Hantavirus in the lungs were identified by real time quantitative fluorescence reverse transcription PCR （Real-Time RT-PCR）. Partial S segments of positive samples were amplified and sequenced with reverse transcription PCR （RT- PCR）. Phylogenetic analysis was used for genotyping. Results Mixed areas were dominated by the Apodemus type in HFRS in Hulunbeier. A total of 362 rodents of 10 species of rodents were captured, and the average density was 13.92% （362/2 600）. The dominant in the field was Apodemus agrarius （43.21%, 70/162） followed by Dalin agrarius （34.57%, 56/162）, and dominant in the house was Rattus norvegicus（40.50%, 81/200） followed by Apodemus agrarius （31.50%, 63/200）. Ninety rat lungs were analyzed by using Real-Time PCR. Hantavirus was detected in 23 samples, and the sub type was HTNV. Partial S segment sequences were recovered from 10 samples and sequenced again.Phylogenetic analysis of partial S segment sequences indicated that all viruses belong to H6 clade. Conclusion Etiological study of rats infected with HFRS in Hulunbeier of Inner Mongolia Autonomous Region is HTNV and the S gene subtype is H6 clade virus.
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2016年第5期373-377,共5页
Chinese Journal of Endemiology
基金
“十二五”国家科技重大专项支撑项目“甘肃及周边省区传染病病原谱流行规律研究”课题资助（2012ZX10004-208）
内蒙古自治区卫生和计划生育委员会科研立项（201301026）
关键词
肾综合征出血热
汉坦病毒
S基因
分型
种系进化
Hemorrhagic fever with renal syndrome
Hantavirus
S gene
Genotyping
Phylogemetic
