摘要
目的:探讨小儿肾虚型哮喘与ORMDL3基因单核苷酸多态性的相关性。方法:128例肾虚型哮喘儿童作为哮喘组,再选择100例健康儿童作为对照组,对两组儿童ORMDL3基因4个单核苷酸多态性位点(SNP)的基因分型分布情况进行分析。结果:ORMDL3基因rs7216389存在C/C、C/T、T/T 3种多态性,在哮喘组患儿中的分布频率分别为3.91%、31.25%、64.84%,在对照组儿童中的分布频率分别为9.00%、43.00%、48.00%,两组基因分布差异有统计学意义(P<0.05);ORMDL3基因rs4378650存在C/C、C/T、T/T3种多态性,在哮喘组患儿中的分布频率分别为49.22%、28.91%、21.88%,在对照组儿童中的分布频率分别为64.00%、22.00%、14.00%,两组基因分布差异具有统计学意义(P<0.05);ORMDL3基因rs807613存在A/A、A/G、G/G3种多态性,在哮喘组患儿中的分布频率分别为60.16%、35.94%、3.91%,在对照组儿童中的分布频率分别为53.00%、40.00%、7.00%,两组基因分布差异无统计学意义(P>0.05);ORMDL3基因rs12603332存在C/C、T/T、C/T3种多态性,在哮喘组患儿中的分布频率分别为58.59%、4.89%、36.72%,在对照组儿童中的分布频率分别为54.00%、9.00%、37.00%,两组基因分布差异无统计学意义(P>0.05)。结论:ORMDL3基因多态性与小儿肾虚型哮喘易感性相关,其中rs7216389以及rs4378650与小儿肾虚型哮喘易感性具有显著相关关系,而rs807613以及rs12603332则与小儿肾虚型哮喘易感性不相关。
Objective：To investigate the correlation of kidney deficiency type asthma in children with ORMDL3 gene single nucleotide polymorphism. Methods：Selected treatment of kidney deficiency type asthma in 128 children as asthma group,and 100 healthy children were selected from the same period in our hospital physical examination as control group,two groups of children of ORMDL3 gene 4 single nucleotide polymorphisms（ SNP） genotyping distribution analysis. Results：ORMDL3 gene rs7216389 had C /C,C / T,T / T three kinds of polymorphism,the frequency distribution of the asthma groups were 3. 91%,31. 25%,64. 84% in the control group,the frequency distribution in children were respectively 9. 00%,43. 00%,48. 00%,with statistical significance of the distribution of the two groups（ P 〈 0. 05）;ORMDL3 gene rs4378650 had C / C,C / T,T / T three kinds of polymorphism,the frequency distribution of the asthma groups were respectively 49. 22%,28. 91% and 21. 88% in the control group,the frequency distribution in children were 64. 00%,22. 00%,14. 00%,with statistical significance of the distribution of the two groups（ P 〈0. 05）;ORMDL3 gene rs807613 had A / A,A / G,G / G three polymorphism,the frequency distribution of the asthma groups were respectively 60. 16%,35. 94% and 3. 91% in the control group,the frequency distribution in children were 53. 00%,40. 00%,7. 00%,the distribution of the two groups was not statistically significant（ P 〉 0. 05）;ORMDL3 gene rs12603332 had C / C,T / T,C / T three kinds of polymorphism,distribution frequency in asthma groups were respectively 58. 59%,4. 89% and 36. 72% in the control group,the frequency distribution in children were 54. 00%,9. 00%,37. 00%,the distribution of the two groups was not statistically significant（ P 〉 0. 05）. Conclusion：ORMDL3 gene polymorphism and susceptibility of children with kidney deficiency type asthma related,in which rs7216389 and rs4378650 and pediatric kidney deficiency type asthma susceptibility has significant correlation,and rs8
出处
《中医学报》
CAS
2016年第4期490-493,共4页
Acta Chinese Medicine
基金
2013年度湖北省黄石市医药卫生科研立项项目{黄科技发农[2013]1号}
关键词
小儿哮喘
肾虚证
ORMDL3基因
单核苷酸多态性
children with asthma
kidney deficiency syndrome
ORMDL3 gene
single nucleotide polymorphism
