摘要
以雷公藤带芽幼嫩茎段为外植体,通过不同的培养基配方,对雷公藤组织培养快速繁殖体系建立进行了研究。试验结果表明:外植体经75%酒精浸泡30 s后,用无菌水冲洗3遍,0.1%升汞溶液浸泡5 min,无菌水冲洗5遍的消毒效果最好,雷公藤组织培养苗的污染率为7.8%;最佳诱导培养基为MS+0.05 mg/L NAA+0.5 mg/L 6-BA,平均速度为3.2 d;最佳继代增殖培养基为1/2 MS+2.0 mg/L 6-BA+1.0 mg/L NAA+25 g/L蔗糖,月增殖系数达5.83;最佳生根培养基为1/2 MS+2.0 mg/L IBA+0.5 g/L AC培养基,生根率达78.3%;最有利于移栽的基质为等容积的沙子+红壤土+泥炭土混合基质,成活率高达87.31%。
By means of different culture media, a tissue culture system of Tripterygium wilfordii was established with its new shoot with buds as explant. Experimental results showed that the most suitable procedure for sterilization was to soak the explants into 0. 1% HgC12 solution for 5 min and then wash with sterile water for 5 times after soaking them into 75% alcohol for 30 s and washing with sterile water for 3 times. The optimum initial medium was MS supplemented with 0. 05 mg/L NAA ＋0. 5 mg/L 6-BA, the optimum subculture medium was 1/2 MS with 2. 0 mg/L 6-BA ＋1.0 mg/L NAA ＋ 25 g/L sucrose, and the optimum rooting medium was 1/2 MS with 2.0 mg/L IBA ＋0.5 g/L AC. The plantlets reached 87.31% survival rate after transplanted in the substrate containing equal volume of sand, red loam and peat soil.
出处
《江苏林业科技》
2016年第2期8-12,16,共6页
Journal of Jiangsu Forestry Science & Technology
基金
国家科技支撑计划项目"雷公藤短葶山麦冬GAP关键技术研究"(2009BAI73B00)
福建省中药材GAP工程技术研究中心资助项目"福建省中药材GAP示范基地的建设"(2008Y2001)
福建省科技重大专项基金资助项目"福建省中药材GAP工程技术研究中心"(2004YZ02-05)
关键词
雷公藤
组织培养
快速繁殖
增殖
Tripterygium wilfordii
Tissue culture
Rapid micropropagation
Proliferation
