摘要
目的:探讨Toll样受体4(Toll-like receptor 4,TLR4)基因3'未翻译区G11367C与NF-κB抑制因子(nuclear factor kappa B,IκB)-αHae III位点多态性的交互作用和急性胰腺炎(acute pancreatitis,AP)及其严重程度的关系。方法:选择新乡医学院第一附属医院2013年5月至2015年6月收治的AP患者450例(AP组),AP组又分为轻度AP组(MAP亚组)、中度AP组(MSAP亚组)和重度AP组(SAP亚组)各150例,以150例健康体检者作为对照组。以上述各组患者的外周血白细胞为样本,利用PCR技术检测TLR4基因3'未翻译区G11367C和IκB-αHae III多态性。每位研究对象进行面对面的问卷调查,采用非条件logistic回归对资料进行分析,估算G11367C和IκB-αHae III多态性与AP发病风险的调整比值比(OR)及95%可信区间(95%CI),并分析G11367C与IκB-αHae III多态性的交互作用。结果:G11367C(GC),IκB-αHae III(AG)和IκB-αHae III(GG)基因型频率AP组的分布分别为69.56%,33.78%和36.22%;MAP亚组分别为49.33%,24.67%和26.00%;MSAP亚组分别为70.67%,34.67%和36.67%;SAP亚组分别为88.67%,42.00%和46.00%;对照组分别为26.67%,14.00%和14.67%;上述基因型频率在AP组与对照组之间以及各AP亚组之间差异均有统计学意义(均P<0.01)。G11367C(GC)基因型者患AP的风险均显著增加(ORAP=6.2828,ORMAP=2.6776,ORMSAP=6.6250,ORSAP=21.5147),IκB-αHae III(AG)和IκB-αHae III(GG)基因型者患AP的风险也显著增加(分别ORAP=5.7369,ORMAP=2.5277,ORMSAP=6.1824,ORSAP=17.85751;ORAP=5.8724,ORMAP=2.5902,ORMSAP=6.4027,ORSAP=18.9022)。基因突变的协同分析发现:G11367C(GC)/IκB-αHae III(GG)基因型者频率在AP组、MAP亚组、MSAP亚组、SAP亚组和对照组的分布频率分别为26.44%,12.67%,26.00%,40.67%和4.00%,AP与对照组之间以及各AP亚组之间差异均有统计学意义(均P<0.01)。G11367C(GC)/IκB-αHae III(GG)基因型者患AP的风险显著增加(ORAP=30.1314,ORMAP=6.7612,ORMSAP=39.5000,ORSAP=401.5833),G11367C(GC)与IκB-αHae III(GG)基因型在AP发生、发�
Objective： To investigate the interaction between polymorphism of Toll-like receptor 4（TLR4） gene G11367 C in 3＇ untranslated region（UTR） and inhibitor of nuclear factor kappa B（IκB）-α Hae III in acute pancreatitis（AP） and the degree of severity.Methods： A total of 450 patients with confirmed AP（AP group）, who came from the First Affiliated Hospital of Xinxiang Medical College from May 2013 to June 2015, were divided into a mild AP subgroup（MAP subgroup）, a moderately severe AP（MSAP subgroup）, and a severe acute AP（SAP subgroup）（n=150 in each group）. One hundred fifty healthy persons were served as a control group. There was no significant difference in age, gender, ethnicity and birthplace among all groups. The genetic polymorphisms of TLR4 gene G11367 C in 3＇ untranslated region and IκB-α Hae III were analyzed by polymerase chain reaction（PCR）. Eligible participants were personally interviewed by a questionnaire. Unconditional logistic regression model and single factor analysis were performed to calculate the adjusted odds ratios（OR） and 95% confidence intervals（95% CI） of G11367 C and IκB-α Hae III polymorphisms, respectively. The interaction of nucleotide polymorphisms was analyzed.Results： The frequencies of G11367C（GC）, IκB-α Hae III（AG） and IκB-α Hae III（GG） were 69.56%, 33.78% and 36.22% in the AP group; 49.33%, 24.67% and 26.00% in the MAP subgroup; 70.67%, 34.67% and 36.67% in the MSAP subgroup; 88.67%, 42.00% and 46.00% in the SAP subgroup and 26.67%, 14.00% and 14.67% in the control group, respectively. There was significant difference in the frequencies betweenc the AP group and the control group, or among each AP subgroup（all P0.01）. The risk of AP was significantly increased in the subjects with G11367C（GC） genotype（ORAP=6.2828, ORMAP=2.6776, ORMSAP=6.6250, ORSAP=21.5147）, which was also increased in those with IκB-α Hae III（AG） genotype（ORAP=5.7369, ORMAP=2.5277, ORMSAP=6.1824, ORSAP=17.8572） and
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2016年第3期272-281,共10页
Journal of Central South University ：Medical Science
基金
河南省教育厅科研基金(2011A320015)~~
