摘要
前期研究表明,玉米大斑病菌Ste12基因对分生孢子发育和致病性有重要的调控作用。本试验利用Ste12基因的RNAi沉默突变体StRNAi 9-10和StRNAi 3-6分析该基因对玉米大斑病菌渗透胁迫的调控能力。通过比较野生型菌株和RNAi沉默突变体在0.4mol/L CaCl2、1mol/L KCl、1mol/L NaCl、1mol/L山梨醇等渗透胁迫条件下的菌落形态、生长速度、菌丝形态、产孢量等指标,发现StRNAi9-10对1 mol/L NaCl和1mol/L KCl胁迫的耐受能力显著增强,对1mol/L CaCl2胁迫的耐受能力显著降低,对1mol/L山梨醇的耐受力无显著差异;StRNAi 3-6对4种胁迫条件的耐受能力均显著降低。以上研究结果表明,Ste12基因不仅调控玉米大斑病菌的分生孢子发育和致病力形成,而且参与玉米大斑病菌的渗透胁迫调控。
Early research had shown that Ste12 gene plays a very important role in conidium development and pathogenicity to corn in Setosphaeria turcica.In this paper,the RNAi silence mutants StRNAi 9-10 and StRNAi 3-6of Ste12 gene were used to analyze the regulation ability of Ste12 gene on osmotic stress of Setosphaeria turcica.By comparing the colony morphology,growth rate,mycelium morphology,and conidial number of the wild type strains and the RNAi silence mutants under different osmotic stress conditions of 0.4mol/L CaCl2,1mol/L KCl,1mol/L NaCl,and 1mol/L sorbitol,it was found that StRNAi 9-10 can markedly enhance its capability of NaCl and KCl stress tolerance,but the capability of CaCl2 stress tolerance is significantly reduced.There is no significant difference for sorbitol tolerance.Under the four kinds of osmotic stress conditions,the tolerance ability of StRNAi 3-6is overall significantly reduced.The research results showed that Ste12 gene could not only regulate theconidial formation and pathogenicity but also plays an important role in osmotic stress regulation of Setosphaeria turcica.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2016年第2期91-95,共5页
Journal of Hebei Agricultural University
基金
国家自然科学基金项目(31271997)
河北省自然科学基金项目(C201405067)
河北省留学人员科技活动项目(C2015005009)
唐山师范学院科学研究项目(2014E04
2013A03)
