摘要
以带菌(E+)和不带菌(E-)的中华羊茅为试材,采用培养皿纸上发芽法,研究了不同NaCl浓度对中华羊茅幼苗苗长、根长、苗鲜重和根鲜重的影响,以期为内生真菌对宿主耐盐性的作用提供参考依据。结果表明:E+幼苗中,盐浓度为1.0-8.0mmol/L时显著促进苗长、根长和苗鲜重的增加(P〈0.05),而1.0-2.0mmol/L盐浓度对根鲜重有明显增效作用(P〈0.05);E-幼苗中,1.0-2.0mmol/L盐浓度显著增加根长(P〈0.05),2.0-8.0mmol/L盐浓度明显增加苗鲜重(P〈0.05),盐浓度8.0mmol/L显著抑制根的伸长和鲜重(P〈0.05)。与E-幼苗相比,对照条件下除内生真菌显著增加苗鲜重外(P〈0.05),其它所测生长指标均无明显差异性;在1.0-8.0mmol/L盐浓度下内生真菌显著增加根长和苗鲜重(P〈0.05),在1.0-4.0mmol/L盐浓度下内生真菌明显促进苗长(P〈0.05),在2.0mmol/L或8.0mmol/L盐浓度下内生真菌明显增加根鲜重(P〈0.05);苗长与根长或苗鲜重相关系数较高,且内生真菌能提高其相关系数。
Using seedlings growth of Festuca sinensis infected-endophyte(E+)and uninfected-endophyte(E-)as material,the effects of NaCl concentrations on seedling length,root length,seedling fresh weight and root fresh weight of Festuca sinensis were studied for providing theoretical basis for the effects of Epichloendophyte infection on salt stress by germination method of Petri plates.The results showed that in E+seedlings salt concentrations ranged from 1.0mmol/L to 8.0mmol/L promoted significantly seedling length,root length and seedling fresh weight(P〈0.05),and salt concentrations between 1.0 mmol/L and 2.0 mmol/L increased significantly root fresh weight(P〈0.05).In E-seedlings root length was increased under salt concentration ranging from 1.0mmol/L to 2.0mmol/L(P〈0.05),seedling fresh weight increased significantly under all salt concentration except 1.0mmol/L NaCl(P〈0.05),and root length and fresh weight were significantly inhibited by 8.0mmol/L NaCl(P〈0.05).Compared with E-seedlings,E+ones had higher seedling fresh weight under control conditions,E+ones had higher root length and seedling fresh weight under all salt concentration(P〈0.05),E+ones had higher seedling length under salt concentration ranging from1.0mmol/L to 4.0mmol/L(P〈0.05),and E+ones had higher root fresh weight under 2.0mmol/L or 8.0mmol/L NaCl(P〈0.05).There were high positive correlation between seedling length and root length,and between seedling length and fresh weight,which were higher in E+seedlings.
出处
《北方园艺》
CAS
北大核心
2016年第9期65-68,共4页
Northern Horticulture
关键词
中华羊茅
内生真菌
盐胁迫
幼苗生长
Festuca sinensis
Epichloё
salt stress
seedling growth