摘要
开发了一种利用微流控芯片选择性地固定和释放单个细胞的方法。该芯片由透明玻璃和有机高聚物PDMS(Polydimethylsiloxane)两种材料组成,利用微加工技术,在芯片内部设计出微米级别的特殊沟道,并结合微气阀的使用,控制芯片内流体的流动状态,从而有效地控制细胞的运动状态,实现细胞选择性地固定和释放。芯片可分为两部分,前部分基于流聚焦的原理合成水凝胶珠并将细胞包裹在水凝胶珠内,对细胞形成保护层,避免操作过程中对细胞造成机械损伤,使细胞保持良好的活性,后部分利用微气阀对包裹有细胞的水凝胶珠进行固定和释放,从而实现细胞的固定和释放。细胞固定后,可对细胞进行实时原位观测,研究细胞的生长过程和对药物的反应。同时,通过调节细胞的浓度,包裹在水凝胶珠里的细胞的个数可以控制为单个水平,从而实现单个细胞的分析。
A new methed was developed to immobilize and release single cell selectively based onmicrofluidic device. The materials of the device were transparent glass and organic polymer PDMS（Polydimethylsiloxane）, micron special channel was designed in the device by micro- machiningtechniques, combined with microvalves, the movement of cells could be effectively controlled byadjusting the flow state of fluid within the device, realizing the immobilization and release of cells. Themicrofluidic device could be divided into two parts, the first part generated hydrogel beads encapsulatingcells based on the principle of flow focusing, the hydrogel beads made a protective shell for cells to avoidmechanical damage caused during operation, which ensured a high cell viability, the second part realizedthe immobilization and release of cells by trapping and releasing the hydrogel beads encapsulating cellsthrough adjusting the microvalves. Therefore in-situ observation on cells could be realized, which wasbeneficial to monitor cell growth and make drug study on cells. Moreover, single-cell analysis could beachieved because the number of cell encapsulated in beads could be controlled in a single lever byadjusting cell concentration.
出处
《科技通报》
北大核心
2016年第5期68-72,共5页
Bulletin of Science and Technology
基金
国家自然科学基金项目(81272443)
国家基础科学人才培养基金(J1210061)
关键词
微流控
微气阀
细胞包裹
细胞的固定和释放
microfluidic
microvalves
cell encapsulation
cell immobilization and release
