摘要
儿童全程接种乙肝疫苗后的应答反应率高（为９６％）［１］；而成人接种乙肝疫苗后免疫应答比较低（为６５．１２％）。本文对成人组接种乙肝疫苗无应答反应１５例（３４．８８％），进一步探索了乙肝疫苗接种后不产生抗－ＨＢｓ的原因，采用聚合酶链反应（ＰＣＲ）技术检测了１５例正常成人经乙肝疫苗接种后无应答反应者和３例乙型肝炎患者的血清，结果发现１５例不产生抗－ＨＢｓ者，经ＰＣＲ扩增ＨＢＶＤＮＡ均为阴性；仅３例为阳性。本文作者在ＰＣＲ检测指导下，对１５例成人采用乙型肝炎免疫球蛋白（ＨＢＩＧ）和３０微克乙肝疫苗联合应用１０５天后，有１２例产生抗－ＨＢｓ。提示对于接种乙肝疫苗的正常人群，凡经ＰＣＲ扩增ＨＢＶＤＮＡ阴性者，经过重复大剂量注射乙肝疫苗后可使绝大多数得到可靠的保护。
The response rate in children after HB vaccination is as high as 96%,while the response rate in adults after that is lower(65.12%).This study is based on 15 adult cases(34.88%)with no response after HB vaccination,trying to determine the cause of the non-production of antiHBs,PCR( Polymerase Catenoid Reaction)test is used to check the sera of 15 normal adults with no response to HB vaccination and 3 patients with HB.After PCR amplifing HBV DNA test the result is positive only in 3 cases and negative in 12 cases. The 15 people were given HBIG and 30 μg HB vaccination, After 105 days,12 cases previously with negative results produced antiHBs,It is suggested that repeated,large amount of HB vaccination can be used on the normal population who have no response to HB vaccination and proved negative by PCR amplifying test , in order for them to abtain reliable protection.
