摘要
目的:甘草种子硬实,表面蜡质包被是影响组织培养条件下甘草种子萌发的主要因素。通过对甘草种子进行处理,得到组织培养条件下提高甘草种子萌发率和消毒效果的方法。方法:将甘草种子用98%硫酸处理、灭菌热水处理、各浓度次氯酸钠溶液处理后在MS培养基上培养,考察种子萌发及消毒效果。结果:组织培养条件下,常温水浸泡24 h的甘草种子吸胀率为36.67%,发芽率为10%,经过浓硫酸浸泡80 min、60℃热水浸泡24 h(自然冷却至室温)和3%次氯酸钠溶液浸泡15 min的甘草种子吸胀率分别达到100%、42.16%和46.7%;发芽率分别为77.79%、36.67%和24.44%。浓硫酸和次氯酸钠溶液处理的甘草种子消毒效果较好。结论 98%硫酸浸泡80 min或者60℃热水浸泡24 h(自然冷却)后再用3%次氯酸钠溶液浸泡15 min,均能使甘草种子达到良好的消毒效果和较高的萌发率。
Objective： The Glycyrrhiza uralensis seeds are difficult to germinate in tissue culture conditions for its hardness and waxiness. Many methods were used to improve the germination rate and disinfection effect of G. uralensis seeds in tissue culture. Methods： The G. uralensis seeds were treated by 98% H2SO4,hot sterile distilled water or sodium hypochlorite solutions,and then cultured on MS medium to investigate the germination rates and disinfection effects. Results： The water absorption rate was 40% and the germination rate was 10% when the G. uralensis seeds were soaked in water at room temperature before cultured on MS medium. However,the water absorption rates reached to 100%,42. 16% and 46. 7%,and the germination rate reached to 77. 79%,36. 67% and 24. 44%,respectively,when the G. uralensis seeds were treated by 98% H2SO4 for 80 min,60 ℃ water for 24 h（ naturally cooled down to room temperature） and 3% sodium hypochlorite. The G. uralensis seeds could be disinfected by 98% H2SO4 and sodium hypochlorite solutions. Conclusion： To improve the germination ratesof G. uralensis seeds in tissue culture,the seeds could be treated by 98% H2SO4 for 80 min or by 60 ℃ water for 24 h（ naturally cooled down to room temperature） followed by 3% sodium hypochlorite surface-sterilization for 15 min.
出处
《中国现代中药》
CAS
2016年第3期339-342,共4页
Modern Chinese Medicine
基金
国家自然科学基金项目(811173488)
中医药行业科研专项(201207002)
中国中医科学院中药研究所项目(2011ZDXK-01)
中国中医科学院中药资源中心项目(KFKT20123005)
广东省中医药局建设中医药强省科研项目(20142090)
关键词
甘草
种子萌发
组织培养
Glycyrrhiza uralensis Fisch.
seed germination
tissue culture
