摘要
目的:探讨microRNA-199a/b-3p(miR199)抑制三阴乳腺癌细胞增殖存活的作用机制。方法:在三阴乳腺癌细胞BT549、MDA-MB-231转染miR199,荧光定量检测miR199表达量;MTT法检测转染miR199对三阴乳腺癌细胞存活率的影响;细胞周期分析检测,转染miR199对在乳腺癌细胞细胞周期的影响。结果:超表达miR199后,三阴乳腺癌细胞BT549、MDA-MB-231的增殖率分别下降(41.02±2.34)%和(28.42±6.70)%,细胞周期均被阻滞在G1期。结论:miR-199a/b-3p抑制三阴乳腺癌增殖,具有抗三阴乳腺癌增殖药物开发的潜质。
Objective： To analyze the inhibiting mechanism of microRNA-199a/b-3p（ miR-199） on cell proliferation of triple negative breast cancer（ TNBC） cells. Methods： Expression of miR199 in BT549 and MDA-MB-231 cells transfected with miR-199 a / b-3p was detected by qRT-PCR. The proliferation of BT549 and MDA-MB-231 cells transfected with miR-199 were analysed by MTT assay. Cell cycle of TNBC cells transfected with miR-199 was detected by Flow Cytometry assay. Results： MiR-199 a / b-3p could suppress the proliferation of BT549 and MDA-MB-231 cells. Comparing with normal control,the proliferation rate were up to（ 41. 02 ± 2. 34） %and（ 28. 42 ± 6. 70） %,and the cell cycle were arrest at G1 phase. Conclusion： MiR-199 a / b-3p could suppress the proliferation of TNBC,and may be a promising anti-cancer drug for TNBC.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2016年第4期480-482,共3页
Chinese Journal of Immunology
基金
吉林大学"大学生创新创业训练计划"创新训练国家级培育项目(No.2015791141)
吉林省科技厅青年基金项目(No.20150520047JH)
吉林省卫生计生科研计划(No.2014Z013)资助
