摘要
背景：胃癌前病变是多种因素共同参与的长期发展过程,骨髓间充质干细胞有修复组织损伤的作用。目的：探讨骨髓间充质干细胞移植对大鼠胃癌前病变的影响。方法：将36只Wistar大鼠随机分为对照组、模型组和移植组。模型组和移植组建立大鼠胃癌前病变模型,移植组大鼠经尾静脉注射1 m L CM-dil标记的骨髓间充质干细胞（3×106个细胞）,每周注射1次,一共3次,模型组和对照组同样方法经尾静脉注入等量的生理盐水。末次注射后1周处死大鼠,观察大鼠胃组织病理变化,检测胃黏膜中血管内皮生长因子的表达以及血清中细胞因子含量。结果与结论：（1）移植组大鼠胃黏膜损伤较模型组轻,移植组血管内皮生长因子表达明显高于模型组和对照组（P〈0.05）;（2）模型组和移植组血清白细胞介素17和γ-干扰素水平高于对照组（P〈0.05）,移植组血清白细胞介素17和γ-干扰素水平低于模型组（P〈0.05）;（3）结果表明,骨髓间充质干细胞通过增加胃黏膜病变部位血管内皮生长因子的表达,降低血清白细胞介素17和γ-干扰素水平,从而提高血管通透性,减轻炎症反应,阻断或者缓解癌前病变的发生。
BACKGROUND： Precancerous lesions are a long-term development process in which many factors are involved. Bone marrow mesenchymal stem cells can repair tissue injury.OBJECTIVE： To investigate the effect of bone marrow mesenchymal stem cell transplantation on gastric precancerous lesions in the rats. METHODS： Thirty-six Wistar rats were randomly divided into control group, model group and transplantation group. Animal models of gastric precancerous lesions were established in the model and transplantation groups. Rats in the transplantation group were given 1 mL of CM-dil-labeled bone marrow mesenchymal stem cells（3×106 cells） via the tail vein, once a week, totally three times. Rats in the model and control group were subjected to the tail vein injection of the same volume of normal saline. Then, rats were sacrificed 1 week after final injection, and pathohistological changes in rat gastric tissue sections were observed. The expression of vascular endothelial growth factor in the gastric mucosa and levels of serum cytokines were detected. RESULTS AND CONCLUSION： The severity of gastric mucosal injury in the transplantation group was lighter than that of the model group. The expression of vascular endothelial growth factor was significantly higher in the transplantation group compared with the model and control groups（P 〈0.05）. The levels of serum interleukin-17 and interferon-γ were significantly higher in the model group than the transplantation group followed by the control group（both P〈 0.05）. Therefore, bone marrow mesenchymal stem cells can increase vascular permeability, reduce inflammation, block or ease the occurrence of precancerous lesions by up-regulating the expression of vascular endothelial growth factor in the gastric mucosa lesions and reducing the expression of interleukin-17 and interferon-γ.
出处
《中国组织工程研究》
CAS
北大核心
2016年第14期2073-2079,共7页
Chinese Journal of Tissue Engineering Research
基金
河南省科技厅基础科技攻关课题(122300450037)~~
