摘要
目的:优化荷泽颗粒中苍术、枳实挥发油的提取及β-环糊精(β-CD)包合工艺。方法:采用水蒸气蒸馏法提取荷泽颗粒挥发油,采用水溶液法制备挥发油β-CD包合物;以包合物收率和挥发油得率为综合评价指标,采用均匀设计试验法考察挥发油与β-CD的比例、水与β-CD的比例、包合温度、搅拌时间对包合工艺的影响,筛选最优工艺;采用显微镜、红外光谱及紫外光谱对所制包合物进行表征。结果:最优包合工艺为挥发油与β-CD比例1∶10(ml/g)、水与β-CD比例10∶1(ml/g)、包合温度30℃、搅拌时间3.5 h。包合物收率为73.06%,挥发油得率达96.24%(RSD≤5.43%,n=3);3项表征试验均证实挥发油已包合于β-CD内。结论:优选的荷泽颗粒中苍术、枳实挥发油的提取工艺及β-CD包合物制备工艺稳定、可行。
OBJECTIVE：To optimize the extraction of volatile oil from Atractylodes lancea and Citrus aurantium and inclusion technology of β-cyclodextrin（β-CD）in Heze granules. METHODS：The steam distillation method was adopted to extract volatile oil from Heze granules,and β-CD inclusion complex was prepared by aqueous solution method. The effects of the ratio of volatile oil to β-CD,the ratio of water volume to β-CD,inclusion temperature and mixing time on inclusion temperature were investigated by uniform design test to screen optimal technology,using the yield of inclusion complex and the yield of volatile oil as index. The inclusion complex was characterized with microscope,IR spectrum and UV spectrum. RESULTS：Optimum inclusion process was as follows as ratio of volatile oil to β-CD 1 ∶ 10（ml/g）,ratio of water to β-CD 10 ∶ 1（ml/g）,inclusion temperature 30 ℃,mixing time 3.5 h;the yield of inclusion complex was 73.06% and the yield of volatile oil was 96.24%（RSD≤5.43%,n=3）. 3 characterization tests indicated that volatile oil had been included in β-CD. CONCLUSIONS：The extraction technology of volatile oil from A. lancea and C. aurantium and inclusion technology of β-CD in Heze granules are stable and feasible.
出处
《中国药房》
CAS
北大核心
2016年第13期1839-1841,共3页
China Pharmacy
基金
湖北省卫生计生委中医药
中西医结合科研重点项目(No.2013Z-Z02)
关键词
荷泽颗粒
挥发油
Β-环糊精
包合物
均匀设计试验
工艺优化
Heze granule
Volatile oil
β-cyclodextrin
Inclusion complex
Uniform design test
Process optimization
