摘要
目的探讨缺氧条件下,Rab11蛋白的表达变化及其在合体滋养细胞胞外体(syncytiotrophoblast exosome,STBEX)过度分泌中的作用。方法将传代培养的Be Wo细胞分为4组(n=24),1Be Wo常氧组:Be Wo细胞在普通培养基、常氧条件下培养;2Be Wo低氧组:Be Wo细胞在普通培养基、低氧条件下培养(92%N2、5%CO2、3%O2);3融合Be Wo常氧组:Be Wo细胞以佛司可林(Forskolin)合体化刺激48 h后,常氧条件下培养;4融合Be Wo低氧组:Be Wo细胞以Forskolin合体化刺激48 h后,低氧条件下培养(92%N2、5%CO2、3%O2)。收集各组细胞上清液并检测STBEX蛋白浓度,采用Western blot检测各组细胞HIF-1α、Rab11蛋白的表达。结果与融合Be Wo常氧组比较,融合Be Wo低氧组上清液中STBEX的蛋白浓度升高,差异具有统计学意义(P<0.05);与Be Wo常氧组比较,Be Wo低氧组上清液中STBEX蛋白浓度升高,差异具有统计学意义(P<0.05);Western blot检测结果:与常氧培养组相比,低氧培养组细胞中HIF-1α与Rab11蛋白表达升高,差异具有统计学意义(P<0.05)。结论缺氧条件下Rab11蛋白可能促进合体滋养细胞胞外体的过度分泌。
Objective To determine the expression of Rab11 and its role in the excessive secretion of syncytiotrophoblast exosome（ STBEX） when subjected to hypoxia. Methods The cultured Be Wo cells were divided into 4 groups,that is,cultured under normoxic condition,under hypoxia（ 92% N2,5% CO2 and 3%O2）,under normoxic conditions after fusioned with Forskolin,and under hypoxia after fusioned with Forskolin. Then the protein concentration of STBEX was measured in the supernatant of every group by BCA protein assay. The protein expression of HIF-1α and Rab11 were examined by Western blotting. Results Compared with the fused Be Wo cells cultured under normoxic condition,the protein concentration of STBEX was significantly elevated in Be Wo fused cells cultured under hypoxia（ P〈0. 05）. The expression levels of HIF-1α and Rab11 were elevated when subjected to hypoxia（ P〈0. 05）. Conclusion Rab11 may promote the excessive secretion of exosome from syncytiotrophoblasts when subjected to hypoxia.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2016年第9期944-948,共5页
Journal of Third Military Medical University
基金
国家自然科学基金青年科学基金(81401224)~~
