摘要
目的:探究整合素α6对模拟微重力下人牙髓干细胞(human dental pulp stem cells,hDPSCs)粘附能力的影响。方法:以PLGA支架为载体,将采用酶消化法培养的hDPSCs接种在PLGA支架上,分普通重力组和模拟微重力组培养72h,Western blot检测整合素α6(Integrinα6)、整合素αv(Integrinαv)、整合素β1(Integrinβ1)、粘着斑激酶(focal adhesion kinase,FAK)、磷酸化粘着斑激酶(phospho-FAK)的达;si-RNA转染hDPSCs,抑制整合素α6表达,DAPI荧光染色检测转染后hDPSCs在PLGA支架上的粘附数量,Western blot检测转染后hDPSCs中整合素β1、FAK、phospho-FAK的表达。结果:模拟微重力下,整合素α6、phospho-FAK蛋白水平上调(P<0.05),整合素αv、整合素β1和FAK的蛋白水平未见显著性差异;si-RNA转染的hDPSCs粘附能力、phosphoFAK蛋白水平均低于对照组(P<0.05),整合素β1和FAK的蛋白水平未见显著性差异。结论:模拟微重力下,hDPSCs在PLGA支架上粘附能力增强可能与整合素α6及其下游信号分子FAK的表达水平上调相关。
Objective：To study the effects of integrinα6on cell adhesion of human dental pulp stem cells seeded on PLGA scaffolds under simulated microgravity.Methods：hDPSCs were seeded on PLGA scaffolds and cultured under the conditions of normal gravity or simulated microgravity for 72 hours.The protein expression level of integrinα6,integrinαv,integrinβ1,FAK and phospho-FAK were measured by Western blot.Integrinα6siRNA was transfected into hDPSCs and DAPI immunofluorescence staining was performed to detect the adhesion ability of hDPSCs.Western blot was conducted to detect the related-protein expression levels.Results：Expression of integrinα6and phospho-FAK were up-regulated in the microgravity group（P〈0.05）compared to control group,while there were no statistical differences in the expression levels of integrinαv,integrinβ1and FAK.Moreover,adhesion ability and phospho-FAK expression of hDPSCs were decreased after transfected with integrinα6siRNA（P〈0.05）,while there were no statistical differences in the expression levels of integrinβ1and FAK.Conclusion：Increased adhesion ability of hDPSCs seeded on PLGA scaffolds may be associated with the up-regulation of integrinα6and its downstream molecule FAK.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2016年第4期361-364,共4页
Journal of Oral Science Research
基金
国家自然科学基金(编号:81271132)
黑龙江省教育厅科学技术研究项目(编号:12531234)
黑龙江省自然科学基金项目(编号:H201440)
关键词
人牙髓干细胞
模拟微重力
细胞粘附
整合素
Human dental pulp stem cells
Simulated microgravity
Cell adhesion
Integrin
