摘要
目的构建胶质瘤细胞系U87的多细胞球体（MCS）模型,探讨不同剂量的X线对胶质瘤多细胞球体上清液基质金属蛋白酶2（MMP-2）活性和水溶性代谢物的影响。方法采用琼脂糖培养法建立U87多细胞球体模型;用0、1、5、10 Gy的X线分别照射多细胞球体,0 Gy组作为对照组,利用高氯酸提取细胞中水溶性代谢物[胆碱（Cho）、肌酸（Cr）、N-乙酰天门冬氨酸（NAA）],采用14.7T高场强磁共振仪获取水溶性代谢物波谱;采用明胶酶谱法测量U87多细胞球体上清中MMP-2活性。结果成功建立了U87多细胞球体模型;多细胞球体在1、5、10 Gy X线照射下的胆碱与肌酸比值（Cho/Cr）分别为2.74±0.02、4.32±0.17、4.92±0.07,与对照组（2.10±0.29）比较,细胞内水溶性代谢物比值Cho/Cr升高（P=0.033,P=0.000,P=0.000）;多细胞球体在0~10 Gy X线照射范围内,随着照射剂量的增加,细胞M M P-2活性增加。与对照组比较,差异有统计学意义（P=0.000）。结论亚致死剂量的X线可导致U87胶质瘤多细胞球体MMP-2活性增高,Cho/Cr升高。
Objective To establish the U87 glioma multicellular spheroid（ M CS） model and to explore the effect of various dosages of X-ray on the activity of M M P-2 and w ater-soluble metabolites of U87 glioma multicellular spheroids.Methods The U87 glioma M CS model w as established and irradiated by X-ray of 0,1,5 and 10 Gy. The 0 Gg group served as the control group. Perchloric acid extracting（ PCA） method w as used to extract w ater-soluble metabolites,including choline（ Cho）,creatine（ Cr）,and N-acetylaspartate（ NAA）,w hich w ere observed using in vitro 14. 7T high resolution ^1H-M RS. The M M P-2 expression in U87 M CS model w as detected w ith gelatin zymography. Results U87 glioma M CS model w as established successfully. With the increase of X-ray doses,the Cho / Cr ratio rose from 2. 74 ±0. 02（ 1 Gy）,4. 32 ± 0. 17（ 5 Gy） to 4. 92 ± 0. 07（ 10 Gy）; compared w ith the control group（ 2. 10 ± 0. 29）,the Cho / Cr ratio increased in the other groups[P = 0. 033（ 1 Gy）,P = 0. 000（ 5 Gy）,P = 0. 000（ 10 Gy） ]. Compared w ith the control group,the activity of M M P-2 also increased（ P = 0. 000）. Conclusion Sublethal irradiation promotes the activity of M M P-2 of U87 M CS and elevates the Cho / Cr ratio.
出处
《山东大学学报（医学版）》
CAS
北大核心
2016年第4期6-10,共5页
Journal of Shandong University:Health Sciences
基金
国家自然科学基金(81372439)
山东省科技发展计划(2014GSF118102)
山东省医药卫生科技发展计划(2014WS0131)
