摘要
斑马纹病是剑麻生产上最严重、毁灭性病害之一。本研究基于寄生疫霉菌多聚半乳糖醛酸酶pppg6~pppg10等5个基因c DNA序列设计了各基因编码区的特异引物。利用5对引物分别对不同来源的剑麻斑马纹病菌DNA进行了分子检测。检测结果表明,在被检测的剑麻斑马纹病菌菌株中均存在与寄生疫霉菌多聚半乳糖醛酸酶pppg6~pppg10对应的基因Szpg6~Szpg10。基因序列比对分析表明,来自斑马纹病菌的Szpg6、Szpg8基因与对应pppg6、pppg8基因之间序列高度一致。比较而言,来自斑马纹病菌的Szpg7、Szpg9以及Szpg10基因与对应同源基因之间分别存在3、6及4个核苷酸序列差异,进而导致3、5及3个推定氨基酸的变异。由此推测,来自斑马纹病菌的Szpg7、Szpg9及Szpg10基因可能与对应基因在功能上存在着一定的差异。本研究为进一步研究剑麻斑马纹病菌多聚半乳糖醛酸酶在致病过程中的作用奠定了基础。
Sisal zebra disease caused by Phytophora nicotianae var. parasitica is a kind of main diseases which can cause serious damage to the sisal. In the present study,five specific primer pairs were designed in coding region based on the reference sequence of pppg6 to pppg10 gene from Phytophora parasitic. Five specific primer pairs were used for the molecular detection and gene cloning from sisal zebra pathgoen DNA. The results showed that Szpg6（ sisal zebra polygalacturonase 6） to Szpg10 genes were conserved in all the tested sisal zebra isolates. Sequence alignment with the reference sequences of pppg6 to pppg10 gene from Phytophora parasitic revealed that 1,3,1,6 and 4 SNPs were identified in the Szpg6 to Szpg10 genes coding regions,respectively. There,five and three of these mutations were nonsynonymous in Szpg7,Szpg9 and Szpg10,and the rest were synonymous. Thus we speculated that there were some differences in function when Szpg7,Szpg9 and Szpg10 genes were compared to pppg7,pppg9 and pppg10. The results have laid a solid foundation for the further study of the role of sisal zebra germs polygalaturonase in the pathogenic process.
出处
《中国麻业科学》
2016年第2期62-68,共7页
Plant Fiber Sciences in China
基金
海南省自然科学基金项目(314105)
现代农业产业技术体系(CARS-19)
中央级公益性科研院所基本科研业务费专项(NO.2014hzs1J012)
中国热带农业科学院环植所自主选题项目(2013hzs JY04)
