摘要
目的分析江西省麻疹病毒分离株的分子生物学特征,掌握江西省目前麻疹病毒流行株的基因特性。方法采用淋巴信号激活因子转染的非洲绿猴肾细胞（Vero/SLAM）对2008年-2012年疑似麻疹患者咽拭子标本进行麻疹病毒分离,分离到的毒株通过逆转录-聚合酶链反应（RT-PCR）方法扩增麻疹病毒血凝素（hemagglutinin,H）基因,并对扩增出的核苷酸序列进行测定,与Gen Bank中麻疹病毒各基因型参考株比较并分析毒株变异情况。结果 2008年-2012年江西省麻疹病毒分离株均为麻疹H1基因型,属于我国麻疹病毒的优势株。13株麻疹病毒分离株之间H基因氨基酸的同源性为98.1%-100.0%;与H1基因代表型China93-7的H基因氨基酸同源性为97.2%-98.1%;与中国疫苗株沪191株的H基因氨基酸同源性为94.8%-95.6%。结论 2008年-2012年江西省麻疹病毒流行株为H1基因型,说明江西省近年来麻疹的流行株仍然是H1基因型。
Objective To analyze molecular biologic characteristics of measles virus isolates,and to master the genetic characteristics of measles epidemic strains in Jiangxi province. Methods Measles virus were isolated from throat swabs of suspected patients with measles from 2008 to 2012 using Vero / SLAM cells transfected lymphatic signal activating factor. And RT- PCR method was employed to amplify H gene fragment of measles isolates for sequencing. Then,the homogeneity of the sequence between these measles virus and the representative strains with each genotype in Gen Bank were analyzed. Results All measles virus isolated in Jiangxi province from 2008 to 2012 belonged to genotype H1 which had been a main genotype containing all of the isolates in China. The isolates shared 98. 1%- 100. 0% identity of amino acid sequence on H gene; when comparing to the virus of H1 genotype strain（ China93- 7）,there were 97. 2%- 98. 1% homogeneties on H gene respectively,and comparing to the China vaccine strain（ Shanghai 191）,there were 94. 8%- 95. 6% homogeneties on H gene respectively. Conclusion Data from phylogenic trees of H gene revealed that the wide- type measles viruses isolated in Jiangxi province during2008- 2012 belonged to genotype H1.
出处
《中国卫生检验杂志》
CAS
2016年第8期1156-1160,共5页
Chinese Journal of Health Laboratory Technology
基金
国家传染病监测技术平台"十二五"重大专项(2012ZX10004207003)
江西省卫生厅科技计划(20113115)
关键词
麻疹病毒
基因型
序列分析
Measles virus
Genotype
Sequence analysis
