摘要
目的观察强化训练对脑卒中后抑郁大鼠抑郁程度及海马区Kalirin一7表达的影响，探讨强化训练改善卒中后抑郁大鼠抑郁程度可能的机制。方法取40只雄性Wistar，采用随机数字法将大鼠分为假手术组、脑卒中后抑郁（PSD）组、普通训练组、强化训练组，每组10只。PSD组、普通训练组、强化训练组采用线栓法建立左侧大脑中动脉（MCAO）模型，并给予慢性不可预知性应激刺激（CMS），假手术组仅分离颈总、颈内、颈外动脉，不做插线处理，也不给予CMS。造模成功后，假手术组与PSD组不进行跑台训练，普通训练组、强化训练组分别进行普通和强化跑台训练。4组大鼠分别于训练前和训练14d、28d后进行行为学实验[糖水消耗实验（SPT）、旷场试验（OFT）、强迫游泳实验（FST）]，然后采用wes[erFlblot法检测其左侧海马区Kalirin一7蛋白的表达情况。结果训练14d后，PSD组、普通训练组、强化训练组各项行为学实验结果与假手术组相比，差异均有统计学意义（P〈0.05），普通训练组的OFT和FST结果分别为（55．71±3．94）S和（67．42±3．64）s，与PSD组相比，差异均有统计学意义（P〈0．05），强化训练组各项行为学实验结果与PSD组和普通训练组相比，差异均有统计学意义（P〈0．05）；训练28d后，PSD组、普通训练组各项行为学实验结果与假手术组相比，差异均有统计学意义（P〈0．05），普通训练组和强化训练组各项行为学实验结果与PSD组相比，差异均有统计学意义（P〈0．05），且强化训练组各项行为学实验结果与普通训练组相比，差异均有统计学意义（P〈0．05）。WesternBlot检测显示，普通训练组、强化训练组海马区Kalirin一7的表达均显著高于PSD组同时间点（P〈0．05）；强化训练组海马区Kalirin一7表达显著高于普通训练组同时间点（P〈0．05）。结论强化训练可
Objective To observe any effect of intensive training on the degree of depression and the ex- pression of Kalirin-7 in the hippocampus of rats modeling with post-stroke depression, and to investigate possible mechanisms. Methods Forty male Wistar rats were randomly divided into a post-stroke depression （PSD） group, a routine training group, an intensive training group and a sham-operation group （n = 10 for each group）. All except the sham-operation group were given the middle cerebral artery occlusion using the intraluminal thread method, and chro- nic mild stress was applied. The PSD group and the sham-operation group were not given any training. The routine training group was trained once a day, and the intensive training group was trained twice a day. The sucrose prefer- ence test, open field test and forced swimming test were conducted at the outset and after 14 and 28 days of training. The expression of Kalirin-7 in the left hippocampus was detected using Western blotting. Results After 14 days of training, significant differences were found in the test results of the sham-operation group compared with the other groups （ P〈0.05）. There were also significant differences in the average results on all 3 tests among all of the groups except the sham-operation group. At both 14 and 28 days after training, the average expression of Kalirin-7 in both the routine and intensive training groups was significantly higher than in the PSD group （P〈0.05） , and that of the intensive training group was significantly higher than that of the routine training group at the same time points （P〈0.05）. Conclusion Intensive training can relieve depression and increase the expression of the Kalirin-7 in the hippocam- pus, at least in rats.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2016年第4期241-245,共5页
Chinese Journal of Physical Medicine and Rehabilitation
基金
山东省自然科学基金（ZR2104JL057）
关键词
强化训练
脑卒中
抑郁
Intensive training
Stroke
Post-stroke depression
