摘要
目的研究不同配伍比例对当归补血汤化学成分和促血管新生作用的影响。方法采用高效液相色谱法测定不同配伍比例当归补血汤中的主要化学成分阿魏酸、毛蕊异黄酮苷、毛蕊异黄酮和芒柄花素含量;采用转基因斑马鱼模型比较不同配伍比例当归补血汤的促血管新生作用。结果随着黄芪含量的增加,不同配伍比例煎液指标成分综合得分有上升趋势,且在黄芪与当归之比为8∶2与5∶1时综合得分最高。对于斑马鱼血管损伤模型,血管恢复率排名前3位的配比分别是黄芪与当归之比为5∶1、6∶1和2∶1。结论从化学成分及促血管新生疗效综合分析,黄芪与当归经典配伍比例(5∶1)好于其他比例。
Objective To study chemical ingredients and angiogenesis effects for different compatibilityproportions of Danggui Buxue Decoction. Methods For chemical components, HPLC was performed to study theingredients （Ferulic Acid, Calycosin-7-glusosido, Calycosin, Formononetin） in Danggui Buxue Decoction. Forangiogenesis effects, transgenosis zebrafish model was incubated with different proportions of Danggui BuxueDecoction. Results With the raise of Astragali Radix content, the synthetic scores of four ingredients in differentcompatibility proportions increased. When the Astragali Radix content to Angelicae Sinensis Radix content was 8 to 2and 5 to 1, the scores were the highest. For zebrafish vascular injury model, vessel recoveries in top three were 5：1,6：1 and 2：1 （Astragali Radix： Angelicae Sinensis Radix）. Conclusion From comprehensive analysis of chemicalcomposition and angiogenesis, the best compatibility ratio of Astragali Radix to Angelicae Sinensis Radix is 5：1.
出处
《中国中医药信息杂志》
CAS
CSCD
2016年第6期83-87,共5页
Chinese Journal of Information on Traditional Chinese Medicine
基金
上海中医药大学预算内科研项目(2013JW36)
"龙医团队
龙医学者"项目(LYTD-14)
关键词
当归补血汤
化学成分
斑马鱼
促血管新生
Danggui Buxue Decoction
chemical components
zebrafish
angiogenesis
