摘要
本研究通过纸上芽床发芽试验,明确不同浓度的Mn处理对带(E+)与不带内生真菌(E-)醉马草(Achnatherum inebrians)种子萌发的抑制作用以及外源水杨酸(salicylic acid,SA)对Mn胁迫的缓解作用。结果表明,与添加Mn处理相比,E+醉马草种子发芽率和发芽指数均显著(P<0.05)高于E-醉马草种子,随着Mn处理浓度的增大,E+醉马草种子的萌发率均显著降低(P<0.05),而E-醉马草种子发芽率和发芽指数无显著差异;随着Mn处理浓度的增加,E+和E-醉马草根长均呈明显的下降趋势,而芽长无显著差异;在外源SA各处理浓度下,E+醉马草根长均显著(P<0.05)高于E-醉马草根长,在10mg·L-1 Mn处理液中分别添加不同浓度的外源SA,结果显示外源SA对Mn处理具有缓解作用。综上所述,内生真菌本身影响醉马草种子的萌发,重金属胁迫对醉马草种子萌发具有一定的抑制作用,而外源SA可以适当地缓解这一胁迫。
Various concentrations of manganese（Mn）and salicylic acid（SA）affected germination of drunken horse grass（Achnatherum inebrians）seeds that were infected with（E＋）or free（E-）of a Epichloe/Neotyphodiumendophyte.The results showed that E＋specimens showed a significantly（P〈0.05）higher germination rate and index than that of under Mn free treatment.The germination rate of E＋specimens showed attend of decreasing on germination rate with the increasing of Mn concentration.There were significant differences existed between treatments（P〈0.05）;but the germination rate and index of Especimens had no significant differences.E＋ specimens showed a significantly（P〈0.05）higher root length when various concentrations of exogenous SA were added.The results showed that exogenous SA could relief the effects of Mn on seed germination.
出处
《草地学报》
CAS
CSCD
北大核心
2016年第1期107-113,共7页
Acta Agrestia Sinica
基金
国家"973"项目(2014CB138702)
国家自然科学基金项目(31372366)
教育部创新团队发展计划项目(IRT13019)资助
