摘要
基因是人类的遗传密码,人类个体之间只有万分之一的基因不相同,却导致了人与人之间丰富的差异。了解这种差异对于科学研究具有巨大的应用价值。PCR是基因研究中常用的手段之一,但传统PCR仪存在反应时间长、能量消耗大、不便于集成与携带等缺陷,微流控技术与PCR结合可以有效缩小反应体系,提高反应效率,且易于集成化与微型化。本文按照微流控PCR芯片的结构分类,详细介绍了微池型、连续流动型PCR芯片,以及电泳、荧光、电化学和DNA杂交阵列等检测方法,并在最后进行了总结与展望。
Gene is the genetic code of human beings,and has only 0. 1‰ difference among individuals but creates a weelthy divesity. Thus it has a huge application value to explore the difference. Polymerase chain reaction（ PCR） is one of the technologies mostly used in genetic research. But traditional thermal cyclers are not only time and energy-consuming,but also difficult to be integrated and portable. The combination of microfluidics and PCR can decrease the reaction volume significantly and increase the reaction efficiency with easy integration and miniaturization. In this review,we briefly introduced microchamber PCR chip and continuous-flow PCR chip according to their structures,and online detection methods including capillary electrophoresis,fluorescence,electrochemistry and DNA hybridization array. At last,we summarized the recent advances and pointed out the future development about microfluidic PCR chip.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2016年第4期542-550,共9页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.21375152)资助~~
关键词
微流控
聚合酶链式反应
芯片
综述
Microfluidics
Polymerase chain reaction（PCR）
Chip
Review
