摘要
【摘要】目的异丙酚复合七氟烷麻醉对大鼠肝脏缺血再灌注损伤的作用及可能机制。方法雄性SD大鼠36只，体重250～300g。按随机数字表法，随机分为A、B、C、D、E、F6个组，每组6只。A、B组采用腹腔注射1％戊巴比妥钠（PB）40mg／kg麻醉，C、D组采用异丙酚复合七氟烷麻醉，E组采用异丙酚麻醉，F组采用七氟烷麻醉。A组仅分离肝脏周围韧带，不阻断入肝血流；B组麻醉后夹闭肝动脉和门静脉左支，造成约70％肝脏缺血60min、再灌注3h，建立肝脏缺血再灌注模型。C组操作同A组，D、E、F组操作同B组。再灌注3h后，取下腔静脉血，检测血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和乳酸脱氢酶（LDH）以7LTNF-α、IL-6含量。结果B组血清ALT、AST、LDH水平明显高于A纽，差异有统计学意义P〈0．05。D、E、F组血清ALT、AST、LDH低于B组，差异有统计学意义（P〈0．05）。D组血清ALT、AST、LDH低于E、F组，差异有统计学意义（P〈0．05）。B纽血清TNF-α、IL-6含量明显高于A组，差异有统计学意义（P〈0．05）。D、E、F组血清TNF-α、IL-6含量低于B组，差异有统计学意义（P〈0．05）。D组血清TNF-α、IL．6含量低于E、F组，差异有统计学意义（P〈0．05）。结论异丙酚复合七氟烷麻醉对大鼠肝脏缺血再灌注损伤具有明显的保护作用，机制可能是通过一定或者不同途径抑制再灌注过程中TNF-α、IL-6等炎性介质的释放，起到肝脏保护作用。
Objective To evaluate the protection and probable mechanisms of propofol-sevoflorane compounded anesthesia on liver ischemia- reperfusion injury in rats. Methods Male Sprague-Dawley rats, weighing 250-300g, were randomly divided into A, B, C, D, E, F 6groups（n=6） using a random number table： 1% sodium pentobarbital intraperitoneal injection anesthesia （60mg/kg） in group A, B, propofol-sevoflorane compounded anesthesia was performde in group C, D, propofol intravenous anesthesia was performed in group E, and sevoflurane inhalation anesthesia in group E group A only hepatoduodenal ligament was separated, porta hepatis did not be blocked up. Hepatic ischemia-reperfusion （ group B ） was established by clamping the hepatic arteries and hilar vessels distributing to the left and median lobes to induce partial hepatic ischemia （ 70% ） for 60 min followed by reperfusion for 3h. group C operation with group A, and group D, E, F operation with group B. The inferior vena blood was harvested 3 h after reperfusion to be detected for serum ALT, AST and LDH activities; plasma levels of TNF-α and IL-6. Results Compared with group A, the levels of ALT, AST, LDH, TNF-α and IL-6 in group B were significantly increased （ P〈0.05 ） . Compared with group B, the levels ofALT , AST, LDH, TNF-α and IL-6 in group D, E, F were decreased （ P〈0.05 ） . Compared with group E, F, the levels ofALT, AST, LDH, TNF-α and IL-6 in group D were decreased （ P〈0.05 ） . The significant change was found in the parameters mentioned above. Conclusion Propofol-sevoflorane compounded anesthesia can significantly protect the liver from ischemia remerfusion injury in rats, the possible mechanism through a certain or various ways to inhibit the release ofTNF-α, IL- 6 during the ischemia reperfusion, thus can protect liver damage.
出处
《浙江临床医学》
2016年第5期830-831,共2页
Zhejiang Clinical Medical Journal
关键词
异丙酚
七氟烷
肝脏
缺血再灌注损伤
炎性介质
Propofol Sevoflorane Liver Ischemia reperfusion injury Inflammatory mediators
