摘要
目的探讨肠道硫化氢(H_2S)合成酶胱硫醚-γ-裂解酶(CSE)与糖尿病(DM)大鼠近端结肠动力的关系。方法一次性腹腔注射1%链脲佐菌素(STZ)65mg/kg,建立1型糖尿病模型,观察大鼠的一般情况;注药第10天制作结肠平滑肌纵行肌条,使用生物机能实验系统记录每组纵行肌肌条自发性收缩活性;分别采用免疫组化及双重免疫荧光的方法观察结肠全层标本及肌间神经丛(MP)中CSE分布;采用Western blot方法观察肠道组织(去黏膜及黏膜下层)CSE的表达。结果 DM组近端结肠肌条收缩活性均低于对照组(P<0.05);CSE在黏膜下层、黏膜下神经丛、MP、纵行肌和环形肌都有分布;CSE在大鼠肌间神经元细胞核中分布,但CSE阳性神经元所占MP比例差异无统计学意义(P>0.05);DM组大鼠结肠(去除黏膜及黏膜下层后)CSE的表达上调(P<0.05)。结论 CSE表达上调可能是DM大鼠近端结肠运动减退的原因之一。
Objective To investigate the relationsheep between colonic H2S-producing enzyme cystathionine-γ-lyase（CSE）and colonic motility in a rat model of diabetes mellitus（DM）.Methods To obtained diabetic rat models,all rats were injected intraperitoneally（ip）1%streptozotocin（STZ,65mg/kg）,type 1diabetes model was established,nornmol conditions were observed periodically.Ten days after treatment,production of colonic longitudinal smooth muscle strips,organ bath recordings were used to test the colonic motility;immunofluorescence and double immunofluorescence lableling method were performed to detect the distribution of CSE;Western blot were performed on rat colonic samples devoid of mucosa and submucosa to detect the expression of CSE.Results DM rats decreased the colonic motility（P〈0.05）;CSE was strongly expressed in the cytosols of the circular and longitudinal smooth muscle cells and the nucleus of the myenteric plexus neurons;CSE distributed in myenteric neurons in the nucleus of rat,but the proportion of MP had no difference（P〉0.05）;DM rats increased the expression of CSE in the colon devoid of mucosa and submucosa（P〈0.05）.Conclusion The decrease of colonic motility in diabetic rat may be associated with the increased production of H2S-producing enzyme CSE.
出处
《重庆医学》
CAS
北大核心
2016年第11期1463-1466,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(81460111)
广西自然科学基金资助项目(2014GXNSFAA118166)
广西壮族自治区卫生厅计划课题(Z2012408)
关键词
硫化氢
糖尿病
胱硫醚-Γ-裂解酶
结肠动力
hydrogen sulfide
diabetes mellitus
cystathionine-γ-lyase
colonic motility
