摘要
滤纸包裹法制备细菌类单细胞生物扫描电镜样品具有耗时短和样品损耗少等优点,不过,样品量较少时,用该方法制样因干燥后难以分辨滤纸上样品位置而造成取样困难,且电镜观察时常常存在较严重的滤纸背景干扰,难以分辨细菌等样品表面的细微结构。为此,笔者对细菌类单细胞样品制备的滤纸包裹法进行了改良,在滤纸包裹的内层衬上1层锡纸,样品涂布于锡纸后再进行脱水等操作。改良后的方法不仅易于分辨样品在锡纸上的分布,方便取样,而且可有效克服原滤纸包裹法制样时产生的背景干扰,能清晰观察细菌样品的表面细微结构,提高了观察效果。
The filter paper pack method is generally used to prepare SEM sample for observation of bacteria and other mono-cell creatures,and it saves time and samples as compared to the traditional method,However,with this method some samples can＇t be distinguished by naked eye in the filter paper after freeze drying. Moreover,the electron micrograph obtained by this method has interference of filter paper background,and it is thus difficult to observe the subtle structure of bacteria on the micrograph. In this context,the filter paper pack was improved by adding a layer of tinfoil onto the filter paper pack as lining,and the samples were dropped on the tinfoil before ethanol dehydration operations. With the improved method the distribution of sample on the foil can be easily distinguished by naked eye without interference of filter paper background,and the subtle structure of bacteria surface can be clearly observed.
出处
《热带生物学报》
2016年第1期124-127,共4页
Journal of Tropical Biology
基金
国家海洋公益性项目(201205025
201405020)
海南省重点科技计划项目(ZDXM20120005)
关键词
扫描电镜
滤纸包裹法
细菌样品制备
方法改良
SEM
filter paper pack method
bacterial sample preparation
improved method
