摘要
目的比较香菇多糖、茯苓多糖、银耳多糖及按一定配比组成的复合多糖对免疫抑制小鼠细胞免疫功能影响。方法采用常规迟发型超敏反应(DTH)动物模型检测T细胞功能,Luminex液相蛋白芯片分析系统检测血清中白介素-2(IL-2)、白介素-6(IL-6)等细胞因子的含量,通过流式细胞技术(FCM)检测T细胞比例及分型,采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)比色法检测T细胞增殖。结果各多糖组小鼠耳肿胀度与模型对照组相比趋于恢复正常,其中香菇多糖组、复合多糖组这一作用更明显,均差异有统计学意义(P<0.05);香菇多糖、茯苓多糖和复合多糖具有上调免疫抑制小鼠血清IL-6水平的作用,均差异有统计学意义(P<0.05);各多糖均有提高小鼠外周血调节性T细胞(Treg)细胞比例的趋势,其中银耳多糖、复合多糖组与模型对照组比较,均差异有统计学意义(P<0.05);各多糖组均有恢复小鼠外周血Th1/Th2(Th1、Th2为2种辅助性T细胞)细胞比例的作用,均差异有统计学意义(P<0.05),其中复合多糖组的这一作用最明显;各多糖均有恢复免疫抑制小鼠脾脏T、B淋巴细胞比例的趋势,其中复合多糖的作用较明显;4种多糖均有促进免疫抑制小鼠脾脏T淋巴细胞增殖的趋势,其中香菇多糖、复合多糖组与模型对照组比较,均差异有统计学意义(P<0.05)。结论由香菇多糖、茯苓多糖和银耳多糖,以及按一定配比组成的复合多糖对免疫抑制小鼠细胞免疫功能均有一定的恢复作用,但复合多糖较单一多糖的作用更明显,显示出复合多糖组分的协同作用。
Objective To compare the different effects of lentinan,pachymaran,tremella polysaccharide and their complex on cellular immunity of immuno-suppressed mice.Methods The function of T cells was detected by delayed type hypersensitivity（DTH）test,and the quantity of cytokines such as IL-2 and IL-6 in serum were detected using Luminex liquid protein microarray analysis system.The ratio and subtype of cells were detected by flow cytometry（FCM）,and the proliferation of T cells was determined by using a colorimetric method with MTT.Results The ear swelling tends to benormal compared with the model group（P〈0.05）,and the lentinan group and polysaccharides complex group had the most significant difference（P〈0.05）.The tremella polysaccharide and polysaccharide complex could upregulated the level of IL-6 in serum of immuno-suppressed mice（P〈0.05）.All the polysaccharides had the tendency of enhancing the ratio of regulatory T cells（Tregs）in blood,the difference in tremella polysaccharide and polysaccharide complex had statistical significance comparing with model group（P〈0.05）.All test samples had function of restoring the ratio of Th1/Th2 cells in peripheral blood in immuno-suppressed mice（P〈0.05）,the function of polysaccharide complex was the most significant among them.All four polysaccharides had the tendency of restoring the ratio of splenic T and B cells in immunosuppressive mice,the most obvious trend of which was polysaccharide complex.All the four polysaccharides had the tendency of promoting proliferation of splenic T cells,the difference of lentinan and polysaccharide complex was statistically significant comparing to the model group（P〈0.05）.Conclusion Lentinan,pachymaran,tremella polysaccharide and their complex could restore the cellular immunity in immuno-suppressed mice,and the complex was the best.
出处
《中国食品卫生杂志》
2016年第2期186-191,共6页
Chinese Journal of Food Hygiene
基金
广东省教育厅科研项目基金资助(2013LYM0014)
关键词
多糖
香菇多糖
茯苓多糖
银耳多糖
免疫抑制
细胞免疫
小鼠
协同作用
Compound polysaccharide
mushroom polysaccharide
poria cocos mushroom polysaccharides
tremella fucifamis berte
immunosuppress
cellular immunity
mice
synergistic effect
