摘要
本研究对重庆荣昌采后发病姜块进行病原菌分离鉴定,分离到一株新的生姜致病菌。通过细菌形态、生理生化、16S r DNA遗传分析鉴定为沙门氏菌(Salmonella enterica)。将沙门氏菌回接姜苗、姜块,发现姜苗叶片失绿,褶皱;姜块表面水渍并有分泌物;动态污染研究表明:沙门氏菌能在姜块中迅速繁殖,显著提高姜块超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)、过氧化氢酶(catalase,CAT)的活性。该研究表明,沙门氏菌能以生姜为寄主存活。
Ginger is an important economic crop with high medicinal and nutritional value. Diseases caused by pathogenic bacteria in ginger have negative impacts on its growth and storage postharvest quality, and often result in significant profit reduction. In the present study, a novel bacterial pathogen, Salmonella enterica, was isolated from postharvest rhizomes in Rongchang, Chongiqng city. The bacterium was identified by 16 S r DNA sequence analysis, and morphological, physiological and bi ochemical tests. After being inoculated with S. enterica, ginger leaves were found to suffer from chlorosis and wrinkle and water and secretion appeared on the surface of rhizomes. Analysis of the population dynamics of S. enterica indicated that the bacterium colonized and grew well on ginger rhizomes. Biochemical analyses indicated that S. enterica activated antioxidant enzyme activities including superoxide dismutase, peroxidase and catalase in host tissues. These results reveal that S. enterica has the capacity to parasitize ginger.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第7期152-156,共5页
Food Science
基金
永川区自然科学基金项目(Ycstc2013nc8009)
重庆文理学院引进人才项目(Z2011RCY02)
国家星火计划项目(2013GA81102)
关键词
生姜
分离
沙门氏菌
抗氧化应答
ginger
isolate
Salmonella enterica
antioxidant response
