摘要
采用硅胶柱层析技术分离制备龙葵果花色苷。分离得到的2个花色苷馏分经紫外-可见光谱、高效液相色谱-电喷雾串联质谱(high performance liquid chromatography electrosprary ionization-mass spectrometry,HPLC-DADESI-MS/MS)进行结构鉴定,并结合酸水解分析糖苷种类。最终确定馏分Ⅰ为飞燕草素-3-琥珀酰阿拉伯糖苷,根据峰面积归一化法计算其纯度为94%;馏分Ⅱ为矢车菊素-3-半乳糖苷和矢车菊素-3-乙酰半乳糖苷,峰面积归一化法计算其纯度分别为45.67%和13.97%。
The objective of this study was to isolate anthocyanins from Solanum nigrum L. fruits by silica gel column chromatography and identify them by UV-visible spectroscopy and high performance liquid chromatography-electrospray ionization tandem mass spectrometry（HPLC-DAD-ESI-MS/MS）. Acid hydrolysis was applied for the identification of glucosides. Three anthocyanins were identified for the first time in Solanum nigrum L. fruits. Only delphinidin-3-succinylarabinoside was detected in fraction I（the purity was 94%）, while both cyanidin-3-galactoside（the purity was 45.67%） and cyanidin-3-acetylgalactoside（the purity was 13.97%） were found to be present in fraction II. The presented method is simple and reliable to isolate and identify the anthocyanins from Solanum nigrum L. fruits.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第7期56-61,共6页
Food Science
基金
黑龙江省卓越农林人才教育培养计划改革试点项目(41110211)
关键词
龙葵
花色苷
分离
鉴定
高效液相色谱-电喷雾质谱联用
酸水解
Solanum nigrum L.
anthocyanins
isolation
identification
high performance liquid chromatography electrosprary ionization-mass spectrometry（HPLC-DAD-ESI-MS/MS）
acid hydrolysis
