摘要
目的初步探讨ADAMl7在人体胎盘绒毛中的表达情况及其对胚胎植入的影响。方法采用半定量RT—PCR和Western blot技术分别检测人体正常孕6、7、8、9周胎盘绒毛及正常未孕分泌期子宫内膜组织中ADAM17mRNA及ADAMl7蛋白的表达情况。结果半定量RT—PCR结果显示胎盘绒毛组织ADAM17mRNA表达明显高于未孕子宫内膜组织，差异有统计学意义（P〈0．01），且在孕9周达到最高；而Western blot结果显示ADAM17蛋白在胎盘绒毛巾的表达及规律与半定量RT—PCR结果基本一致，差异无统计学意义（P〉0．05）。结论胎盘绒毛组织ADAM17在胎盘绒毛中能够成功表达，可能在促进人体成功的胚胎植入过程中发挥着重要作用。
Objective To investigate the expression of ADAMI7 in human placental villus and its effect on embryo implantation. Methods Semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） and Western blot were applied to detect the expression of ADAM17mRNA and the protein ADAM17 in villus tissues of normal pregnant woman and normal non-fertile endometria respectively at week 6,7,8,9 in the secretive phase. Results Semi-quantitative RT-PCR detection results indicated that the expression level of ADAM17mRNA in villus was obviously higher than that of the non-fertile endometria, with statistical significance（P 〈 0.01 ）. Furthermore, its expression level reached peak at week 9 after pregnancy. The expression pattern and level of ADAM17 by Western blot detection was generally identical to that by semi- quantitative RT-PCR, and no statistical significance could be noted, when comparisons were made between the two（P 〉0.05）. Conclusions ADAM17 could be successfully expressed in placental villus and it might play an important role in the process of embryo implantation.
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2016年第1期53-56,共4页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
