摘要
目的研究鲑鱼降钙素（SCT）在Caco-2细胞模型的吸收机制。方法采用人源结肠癌细胞系Caco-2细胞模型研究不同浓度SCT由绒毛面（AP侧）到基底面（BL侧）的转运过程,用HPLC法测定SCT在转运液中的浓度。结果 SCT 0.5~1.5 mg·m L-1对其表观渗透系数（Papp）无显著影响。结论 SCT在小肠上皮细胞主要以被动转运的方式吸收,有望将其开发成生物利用度较高的口服给药系统。
OBJECTIVE To study the absorption mechanism of salmon calcitonin by Caco- 2 monolayer model. METHODS A Caco- 2（ the human colonic adenocarcinoma cell lines） cell monolayer had been applied to study the transport process of Salmon calcitonin from apical side（ AP） to basolateral side（ BL）. The concentration in transfport medium buffer was performed by HPLC.RESULTS The results indicated that when SCT concentration between 0. 5 to1. 5 mg·m L- 1,there was no significant difference of apparent permeability coefficients（ Papp）. CONCLUSION The passive transference is the main intestinal absorption mechanism of salmon calcitonin in the Caco- 2 monolayer model. Salmon calcitonin is expected to develop a good oral delivery system.
出处
《华西药学杂志》
CAS
CSCD
2016年第2期154-156,共3页
West China Journal of Pharmaceutical Sciences
基金
成都市企业自主创新项目(11GGY13529SW-289)
