期刊文献+

下丘脑兴奋潜能增加介导孕期摄食限制所致子代雌性大鼠HPA轴高敏感性宫内编程

Prenatal Food Restriction Programed the Hypersensitivity of the HPA Axis in Female Offspring Rats Through Stimulating the Hypothalamic Irritability
原文传递
导出
摘要 目的:探讨下丘脑局部兴奋性在孕期摄食限制(PFR)所致子代雌性大鼠下丘脑-垂体-肾上腺(HPA)轴高应激敏感性编程中的作用。方法:建立大鼠PFR模型,孕20d处死部分母鼠取胎鼠。余自然生产,断奶后给予持续高脂饮食饲养,17周时处死一半动物。余于17-20周持续给予不可预测性慢性刺激(UCS)后处死。取雌性动物血清和下丘脑,检测相关指标。结果:PFR组下丘脑超微结构出现损伤,AVP、GAD65、vGLUT2mRNA水平降低,vGLUT2/GAD65比值升高(P<0.05)。UCS前,PFR组血清ACTH浓度、下丘脑CRH mRNA水平降低,vGLUT2/GAD65比值升高(P<0.05)。UCS后,PFR组血清ACTH增长率及下丘脑AVP mRNA水平、vGLUT2/GAD65比值均高于对照组(P<0.05)。结论:PFR诱导的宫内下丘脑局部高兴奋性参与了子代雌性大鼠成年后HPA轴高应激敏感性编程。 Objective:To investigate the role of hypothalamic irritability induced by the prenatal food restriction(PFR)in the stimulated sensitivity of the hypothalamic-pituitary-adrenal(HPA)axis in female offspring rats.Methods:PFR model was established by reducing 50%food administration of the pregnant rats since pregnant day 11.Then,the rats were killed under anaesthesia at pregnant day 20.The left pregnant rats were kept until spontaneous labor.All the female offspring were fed with high fat diet.Then,half of the rats were killed,while the left were underwent unpredictable chronic stresses(UCS)from postnatal week 17 to 20and killed as well.The bloodand hypothalamus were collected.Serum adrenocorticotropic hormone(ACTH),hypothalamic mRNA level of hypothalamus corticotrophin-releasing hormone(CRH),arginine vasopressin(AVP),vesicular glutamate transporter 2(VGluT2),glutamic acid decarboxylase 65(GAD65),and ultrastructure of fetal hypothalamus were detected.Results:Abnormal changes in ultrastructure were observed in fetal hypothalamus.The mRNA levels of AVP,GAD65,vGLUT2 were decreased,while the vGLUT2/GAD65 ratio was increased.Before UCS,lower ACTH serum concentration and CRH mRNA level,as well as increased vGLUT2/GAD65 ratio,were detected in PFR group.Then,the rate of the serum ACTH rise,the AVP mRNA level and vGLUT2/GAD65 ratio were elevated after UCS.Conclusion:PFR stimulated the sensitivity of the HPA axis in female offspring rats through boosting the hypothalamic irritability.
出处 《武汉大学学报(医学版)》 CAS 2016年第3期345-349,共5页 Medical Journal of Wuhan University
基金 国家自然科学基金重点项目(编号:81430089) 重大合作项目(编号:81220108026)
关键词 孕期摄食限制 下丘脑 下丘脑-垂体-肾上腺轴 高应激敏感性 宫内编程 Prenatal Food Restriction Hypothalamus Hypothalamic-Pituitary-Adrenal Axis Irritability Intrauterine Programming
  • 相关文献

参考文献11

  • 1Zhang C,Xu D,Luo H,et al.Prenatal xenobiotic exposure and intrauterine hypothalamus-pituitary-adrenal axis programming alteration[J].Toxicology,2014,325:74-84. 被引量:1
  • 2Barker DJ.The developmental origins of chronic adult disease[J].Acta Paediatr Suppl,2004,93(446):26-33. 被引量:1
  • 3Vieau D,Sebaai N,Leonhardt M,et al.HPA axis programming by maternal undernutrition in the male rat offspring[J].Psychoneuroendocrinology,2007,32(Suppl 1):16-20. 被引量:1
  • 4Desai M,Babu J,Ross MG.Programmed metabolic syndrome:prenatal undernutrition and postweaning overnutrition[J].Am J Physiol Regul Integr Comp Physiol,2007,293(6):2 306-2 314. 被引量:1
  • 5Gohlke JM,Griffith WC,Faustman EM.A systemsbased computational model for dose-response comparisons of two mode of action hypotheses for ethanol-induced neurodevelopmental toxicity[J].Toxicol Sci,2005,86(2):470-484. 被引量:1
  • 6Armario A.Activation of the hypothalamic-pituitaryadrenal axis by addictive drugs:different pathways,common outcome[J].Trends Pharmacol Sci,2010,31(7):318-325. 被引量:1
  • 7Herman JP,Mueller NK,Figueiredo H.Role of GABA and glutamate circuitry in hypothalamo-pituitaryadrenocortical stress integration[J].Ann N Y Acad Sci,2004,1 018:35-45. 被引量:1
  • 8Flak JN,Ostrander MM,Tasker JG,et al.Chronic stress-induced neurotransmitter plasticity in the PVN[J].J Comp Neurol,2009,517(2):156-165. 被引量:1
  • 9Zhang L,Xu D,Zhang B,et al.Prenatal food restriction induces a hypothalamic-pituitary-adrenocortical axis-associated neuroendocrine metabolic programmed alteration in adult offspring rats[J].Arch Med Res,2013,44(5):335-345. 被引量:1
  • 10Tegethoff M,Pryce C,Meinlschmidt G.Effects of intrauterine exposure to synthetic glucocorticoids on fetal,newborn,and infant hypothalamic-pituitary-adrenal axis function in humans:a systematic review[J].Endocr Rev,2009,30(7):753-789. 被引量:1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部