摘要
目的探讨老年人CD71＋CD235a＋有核红细胞的免疫功能特点及可能涉及的机制。方法分别取健康老年人及青年人外周血，通过流式细胞仪测定各组CD71＋CD235a＋有核红细胞占外周血单个核细胞（PBMc）的比例；通过流式分选法分选获得各组有核红细胞，磁珠分选法获得T细胞，T细胞增殖实验分3组进行：老年组：老年有核红细胞＋经羟基荧光素-醋酸盐琥珀酰亚胺脂（CFSE）染色的T细胞；青年组：青年人有核红细胞＋经CFSE染色的T细胞；对照组：经CFSE染色的T细胞；实时荧光定量PCR法比较青年组及老年组有核红细胞表达免疫抑制性细胞因子的差异。结果（1）老年人有核红细胞占PBMC比例高于青年人[（9．93±2．95）％和（1．96±1．16）％，t=3．37，P〈0．001]；（2）老年组有核红细胞能显著抑制T细胞的增殖（t=2．91，P〈0．05），而青年组并没有这一现象（t=0．387，P〉0．05）；（3）与青年组相比，老年组有核红细胞能分泌更多量的精氨酸酶（Arg）-2（t=9．04，P〈0．01）、白细胞介素（IL）-1β、IL-6和转化生长因子（TGF）-β（t=4．51、5．46、6．92，均P〈0．05）。结论老年人外周血有核红细胞占外周血单个核细胞比例较青年人高；老年人外周血有核红细胞可能通过释放更多量的Arg-2，IL-1β，IL-6和TGF-β来抑制T细胞的增殖，从而发挥免疫抑制的作用。
Objective To explore the immune property and possible mechanism of the CD71＋ CD235a＋ nucleated erythroid cells from peripheral blood in elderly, as compared with those of healthy young. Methods Peripheral blood obtained by venipuncture from healthy young（n=59,mean age= 28 years）and elderly（n= 78, mean age= 68 years）donors were measured by flow cytometry to evaluate the frequency of the CD71＋ CD235a＋ cells in peripheral blood mononuclear cells （PBMC）. In vitro assays,CD71＋ CD235a＋ cells were sorted by flow eytometry and T cells were sorted using CD4＋ T cell isolation kit,then the T cell proliferation assays were conducted by following groups：T cell ＋ CFSE group;T cell ＋CFSE co-cultured with CD71＋ CD235a＋ cells from the young donors;T cell ＋ CFSE co-cultured with CD71＋ CD235a＋ cells from the older donors. Real-time PCR were used to identify the expression of the immune cytokine secreted by CD71＋ CD235a＋ cells. Results （1）the results showed a significant increase in the percentage of CD71＋ CD235a＋ cells in the elderly compared with the young [（9.93± 2.95）% vs （1.96± 1. 16 ）% , t = 3.37, P〈 0. 001]; （2）the CD71＋ CD235a＋ cells from the older donors could suppress the proliferation of the T cells （t = 2.91, P〈0.05）while from the young group we could not observe this phenomenon（t= 0. 387, P〉0.05）. （3）The results of Real-time PCR revealed that, compared with CD71＋ CD235a＋ cells from the young, CD71＋ CD235a＋ cells from the elderly expressed higher levels of Arg-2（t= 9.04,P〈0.01）,IL-1β ,IL-6 and TGF-β（t= 4.51,5.46, 6.92,all P〈0.05）. Conclusions There are higher frequencies of CD71＋ CD235a＋ cells in aged samples than the young. And the CD71＋CD235a cells from the elderly could secrete more Arg-2, IL- 1β,IL-6 and TGF-β to suppress the T cell proliferation.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2016年第4期417-420,共4页
Chinese Journal of Geriatrics
基金
江苏省自然科学基金（BK2012607）
苏州市科技发展项目（SYS201116）
关键词
抑制因子
免疫
淋巴细胞
红细胞
Suppressor factors, immunologic
Lymphocytes
Erythrocytes
