摘要
目的:探讨雌激素对阿尔茨海默病(Alzheimer’s disease,AD)模型大鼠学习记忆能力的影响。方法:选取雌性SD大鼠24只,随机分为假手术组、卵巢切除组(ovariectomy,OVX)、OVX+苯甲酸雌二醇组(estradiolbenzoate,EB),每组8只。于海马注射Aβ1-42建立AD大鼠模型,通过Morris水迷宫观察大鼠的学习记忆能力,同时用ELISA检测脑组织超氧化物歧化酶(super oxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、乙酰胆碱酯酶(acetylcholine esterase,ACh E)的活性,用免疫组化分析神经元型一氧化氮合酶(n NOS)并测定其OD值。结果:与OVX组比较,OVX+EB组逃避潜伏期明显缩短(P<0.05),原平台象限活动时间明显增加(P<0.05),穿越原平台次数明显增多(P<0.05)。雌激素作用还提高大鼠脑组织SOD、ACh E和n NOS活性,降低MDA活性(P<0.05)。结论:研究表明雌激素可改善AD模型大鼠的学习记忆能力,其机制可能通过提高脑组织SOD、ACh E和n NOS活性,降低MDA活性有关。
Objective： To evaluate the effect of estrogen on learning and memory ability in model rats with Alzheimer＇s disease（ AD）. Methods： Twenty-four female SD rats were used and were randomly divided into the sham operation,ovariectomy（ OVX） and OVX ＋ estradiolbenzoate（ EB） groups of eight each. AD rat models were established by injection of amyloid beta peptide 1-42（ Aβ1-42） into the hippocampus of rats,then learning and memory ability was assessed by Morris water maze,and activity of super oxide dismutase（ SOD）,and malondialdehyde（ MDA） and acetylcholine esterase（ACh E） were tested by ELISA,and neuronal nitric oxide synthase（n NOS） was detected by immunohistochemal staining and the related optical density（ OD） was examined. Results： Compared with the OVX group,the escape latency was significantly shorter（ P〈 0. 05）,the active time in the original platform quadrant was significantly increased（ P〈 0. 05）,and the number of times of crossing the original platform was significantly increased（ P〈 0. 05） in the OVX ＋ EB group. The activities SOD,Ach E and n NOS were heighted,and the MDA content was decreased（ P〈 0. 05）. Conclusion： The present study showed that estradiol benzoate could improve the learning and memory ability of AD rats,and the mechanism may be related to the increased activity of SOD,ACh E and n NOS,and the decreased activity of MDA in brain tissue of AD rats.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2016年第2期241-245,共5页
Chinese Journal of Neuroanatomy
基金
河北省医学科学研究重点课题计划项目(20150215)
