摘要
为了研究藏药大花绿绒蒿内生真菌Neurospora sp.DHLRH-F的次级代谢产物,采用硅胶、C-18反相硅胶、Sephadex LH-20等柱色谱及HPLC技术对其发酵物进行了系统分离;采用波谱分析技术进行化合物结构鉴定;采用光度法研究所得化合物对Fe2+,Fe3+的络合能力。结果从大花绿绒蒿内生真菌Neurospora sp.DHLRH-F的发酵物中分离得到3个多羟基化合物,分别鉴定为2-氯-6-甲氧基间苯二酚(1)、6-epi-5'-hydroxy-mycosporulone(2)、霉酚酸(3)。本文是首次关于绿绒蒿属植物内生真菌次级代谢产物研究的报道;分离得到的化合物1是新天然产物,有一定的络合Fe3+能力。
To study the secondary metabolites of endophytic fungus Neurospora sp. DHLRH-F from Meconopsis grandis Prain from Tibet,its fermentation products were isolated and purified by silica gel,Sephadex LH-20,ODS-18 column chromatography,and HPLC. The structures of obtained compounds were identified by NMR and MS analysis,and the absorption spectrums of the compounds-iron（ Ⅱ） or iron（ Ⅲ） were investigated by UV / Vis spectroscopy,respectively. The results showed that three polyphenols were isolated from the fermentation compounds of Neurospora sp. DHLRH-F. Their structures were identified as 2-chloro-6-methoxybenzene-1,4-diol（ 1）,6-epi-5＇-hydroxy-mycosporulone（ 2）,and mycophenolic acid（ 3）; compound 1 was a new natural product and showed a certain complexation ability for iron（ Ⅲ）. We firstly reported about secondary metabolites of endophytic fungus from Meconopsis Vig..
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第2期81-84,共4页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
西藏自治区科技计划重点资助项目(毛瓣绿绒蒿等濒危藏药材内生菌次级代谢及其与宿主互惠共存关系研究(2011))
国家自然科学基金地方科学基金资助项目(21262033)
