摘要
目的：研究柴胡注射液解热作用的谱-效关系。方法：采用高效液相色谱（HPLC）法建立柴胡注射液的指纹图谱,测定10批柴胡注射液对干酵母致热大鼠的解热作用,筛选指纹图谱主峰与不同时点（0、10、15、30、40、55、70 min）温度值。采用主成分法提取主成分,依托主成分相关系数矩阵,分析指纹图谱与降温效果间的间接关系。结果：指纹图谱共得到39个共有峰（相似度大于0.85）,将峰面积较大的8、12、14、19、26、31、34、35、39号共有峰纳入研究。主成分分析法提取出4个主成分（占总变异的87%以上）,第1主成分提示12号峰所代表的有效成分可能与6~13 h的降温效果有关;第2主成分提示第26号峰代表的有效成分可能与0.5~5 h的降温效果有关;第3主成分提示第34、35、39号峰可能由能产生相似效应的有效成分引起的;第4主成分提示第14号峰与31号峰可能存在某种共性。结论：柴胡注射液对发热模型大鼠有改善作用,HPLC指纹图谱与柴胡注射液解热作用有较强相关性。
OBJECTIVE：To study the spectrum-effect relationship of the antipyretic effect of Radix Bupleuri injection. METHODS：HPLC method was used to establish the fingerprint of Radix Bupleuri injection. The antipyretic effect of 10 batches of Radix Bupleuri injection on fever rats induced by dried yeast were determined respectively. The fingerprint peaks were screened,along with the temperature value of different time points（0,10,15,30,40,55,70 min）. The principal components were extracted by principal component method and the indirect relationship between the fingerprint and antipyretic effect was analyzed,depending on the principal component correlation coefficient matrix. RESULTS：There were 39 common peaks in fingerprint（similarity0.85）,No. 8,12,14,19,26,31,34,35 and 39 common peaks with large peak area were included in the study. Four principal components were extracted by principal component analysis（87% of total variant）. First principal component showed that the active components of the 12 th peak may be related to the antipyretic effect of 6 to 13 hours. The second principal component showed that the active components of the 26 th peak may be related to the antipyretic effect of 0.5 to 5 hours. The third principal component showed that the similar effect of the active components could be caused by 34 th,35th and 39 th peaks. The fourth principal component suggested that there were some similarities between the 14 th and the 31 st peaks. CONCLUSIONS：Radix Bupleuri injection have obvious improvement for fever rats. There is certain corresponding relation between HPLC fingerprint and antipyretic effect of Radix Bupleuri injection.
出处
《中国药房》
CAS
北大核心
2016年第10期1340-1343,共4页
China Pharmacy
基金
成都医学院2013年度科研项目(No.CY213-009)
关键词
柴胡注射液
指纹图谱
解热
谱-效关系
大鼠
Radix Bupleuri injection
Fingerprint
Antipyretic effect
Spectrum-effect relationship
Rat
