摘要
目的 探讨人类乳头状瘤病毒（HPV） 16 E7蛋白在喉鳞状细胞癌中的表达情况及其对预后的影响.方法 应用组织芯片技术及免疫组织化学方法检测152例喉鳞状细胞癌、40例喉癌前期病变以及40例声带息肉患者组织中HPV16 E7蛋白的表达情况,结合临床相关因素进行统计分析.结果 HPV16 E7蛋白在声带息肉中无表达,癌前期病变以及喉癌组织的阳性表达率分别为2.5％、32.9％.喉癌的不同病理分级、发生部位、临床分期及淋巴结转移之间的HPV E7蛋白的表达差异无统计学意义（P＞0.05）.E7阳性喉癌组5年总体生存率为79％,E7阴性的喉癌组5年总体生存率为66％,两者之间差异无统计学意义（P=0.581）;E7阳性和E7阴性的喉癌患者5年无病生存率分别为57.8％和42.3％,两者之间差异无统计学意义（P=0.579）.Cox比例风险模型回归分析显示E7蛋白不是影响喉癌预后的因素.结论 E7蛋白在喉鳞状细胞癌有一定表达.E7蛋白的表达与喉癌的预后无明显相关性.
Objective To explore the role of human papillomavirus （HPV） in squamous cell carcinoma of the larynx.Methods A cohort of 152 laryngeal squamous cell carcinoma （LSCC） patients,40 premalignant lesions of the larynx,and 40 vocal fold polyp patients was evaluated for the expression of HPV16 E7 protein by immunohistochemistry （IHC）.E7 protein expression was correlated with clinicopathological features and outcomes.Results The expression of E7 protein in vocal fold polyp was negative,and it＇s positive expression in precancerous lesions and laryngeaI carcinomas was 2.5%,and 32.9%,respectively.There were no other significant clinical or demographic differences between pl6-positive and-negative cases.There was no difference in overall survival （OS） between E7-positive and-negative patients with 5-year survival of 79% and 66% for p16 positive and negative patients,respectively （P =0.581）.There was no statistically significant difference in failure-free survival （FFS） with 5-year FFS of 57.8% and 42.3% for p16 positive and negative patients,respectively （P =0.579）.Conclusions E7 expression is infrequent in laryngeal carcinomas.There are no statistically significant correlations between E7 IHC status and OS or FFS.
出处
《中国医师杂志》
CAS
2016年第3期380-383,387,共5页
Journal of Chinese Physician
基金
海南省卫生和计划生育委员会2014年度普通医学科研资助项目（54）
