摘要
目的探讨骨髓间充质干细胞(BMSCs)在大鼠颌下腺细胞放射性损伤中的修复作用。方法采用60钴照射,建立大鼠颌下腺细胞损伤模型,将BMSCs与颌下腺细胞共培养。激光共聚焦显微镜下观察细胞形态,采用MTS法检测细胞增殖情况。结果 60钴照射5min可对体外培养的颌下腺细胞造成实验所需的放射性损伤。共培养细胞中部分BMSCs呈现颌下腺腺泡细胞形态,共培养细胞的增殖率高于经过放射性损伤的颌下腺细胞(P<0.01),而与正常颌下腺细胞和BMSCs相比无统计学差异(P>0.05)。结论 BMSCs具有转化为颌下腺腺泡细胞的潜力,对放射性涎腺细胞损伤起到一定的修复作用。
Objective To investigate the rapair effect of bone marrow mesenchymal stem cells（BMSCs）on the radioactive damage of submandibular gland cells in rats.Methods The damage model of submandibular gland cells was established by ^60Co irradiation for different times.The BMSCs then were co-cultured with submandibular gland cells.The cell morphology was observed under laser scanning confocal microscope and cell proliferation was detected by MTS assay.Results The expected radioactive damage of submandibular gland cells in vitro has been successfully established by ^60Co irradiation for 5 minutes.Part of BMSCs in co-cultured cells appeared the morphology of submandibular gland acinar cells.The proliferation rate of co-cultured cells was higher than that of submandibular gland cells after irradiation（P〈0.01）,but was not significantly different from that of normal submandibular gland cells and BMSCs（P〉0.05）.Conclusion BMSCs have the potential to transdifferentiate into submandibular gland acinar cells and play a recovery role in the radioactive damage of submandibular gland cells.
出处
《江苏医药》
CAS
2016年第6期625-627,F0003,I0001,共5页
Jiangsu Medical Journal
关键词
骨髓间充质干细胞
颌下腺细胞
放射性损伤
Bone marrow mesenchymal stem cells
Submandibular gland cells
Radioactive damage
