摘要
采用单因素试验和正交试验研究超声辅助碱液提取变叶海棠总黄酮的最优工艺条件,以料液比、超声温度、功率、时间为考察因素,以黄酮提取率为指标,并测定了最优条件下的提取物抗氧化活性。结果表明,最优工艺条件为液料比60∶1 m L/g,超声温度35℃,超声时间8 min,超声功率126.5 W。最优工艺条件下黄酮提取率可达13.1432%。抗氧化分析表明,提取物有较强的还原力,清除DPPH自由基的IC_(50)值为2.39 mg/L,清除羟基自由基的IC_(50)值为0.45 mg/L,且活性大小与黄酮的质量浓度呈明显的量效关系。
In this study, total flavonoids from Malus torigoides were ultrasonically extracted by alkaline water. The effects of solid to liquid ratio, ultrasonic temperature, power and time on the extraction yield of flavonoids were investigated and these parameters were optimized using single factor and orthogonal experiments. In addition,the content and antioxidant activity of the extracted total flavonoids were determined. The results showed that the optimal extraction conditions were solid to liquid ratio of 60：1 mL/g,ultrasonic temperature of 35 ℃, ultrasonic time of 8 min, ultrasonic power of 126.5 W. Under the optimized conditions,the content of flavonoids was 13. 1432%. Antioxidant analysis showed that the ex- tract had strong reducibility and scavenging capacity against DPPH free radicals with an IC5o value of 2.39 mg/L and hydroxyl free radicals with an IC50 value of 0.45 mg/L, and these activities were concentration dependent.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2016年第3期452-456,461,共6页
Natural Product Research and Development
关键词
变叶海棠
总黄酮
超声提取
抗氧化
Malus toringoides
flavonoids
ultrasonic extraction
antioxidant activity
