摘要
目的对深圳市罗湖区急性呼吸道病毒感染病例样本进行病原学检测,分析呼吸道病毒的流行特征,为呼吸道感染的预防控制提供科学依据。方法收集辖区哨点医院呼吸道感染病例鼻咽拭子标本,采用RT-PCR方法检测7种常见的呼吸道病毒共计15个亚型：流感病毒（flu A、flu B）、呼吸道合胞病毒（RSVA、RSVB）、鼻病毒（HRV）、腺病毒（ADV）、副流感病毒（PV1-PV3）、人偏肺病毒（hm PVA、hm PVB）、冠状病毒（Co V-HKU1、Co V-229E、Co V-OC43、Co V-NL63）。结果 281份呼吸道感染病例样本,病毒检出阳性率为48.04%（135/281）,其中以流感病毒检出率最高（28.11%）,其次是鼻病毒（7.83%）,2月-5月为呼吸道病毒感染的高峰期,0岁-病毒检出率最高。结论流感病毒是罗湖区最常见的呼吸道病原体,其他呼吸道病原体多样化特征不明显。婴幼儿对呼吸道病毒最为敏感,是防治工作的重点。
Objective To make the pathogenic detection on the respiratory viruses of respiratory tract infections in Luohu district,Shenzhen,and analyze the epidemiological characteristics of it,so as to provide scientific evidence for the prevention and control. Methods Collecting nasopharynx- swab samples of respriatory virus infected cases from sentinel hospitals in Luohu district of Shenzhen,seven kinds of common respiratory viruses were detected by RT- PCR,including fifteen sreotyes： Flu（ A,B）,respiratory syncytial virus（ RSV- A,RSV- B）,rhinovirus（ HRV）,adenovirus（ ADV）,parainfluenza virus（ PV1-PV3）,human metapneumovirus（ hm PVA,hm PVB）,coronavirus（ Co V- HKU1,Co V- 229 E,Co V- OC43,Co V- NL63）.Results Among all the 281 samples,the total positive rate was 48. 04%（ 135 /281）,with Flu- positive rate as the highest（ 28. 11%）,followed by rhinovirus（ 7. 83%）. The respiratory most occurred during February- May,and the positive rate was the highest in patients aged 0- 5. Conclusion Flu is the major respriatory virus of influenze- like illness in Luohu district of Shenzhen,with other respiratory viruses＇ diversity not obvious. Infants were the most easy- infected group and also the focus of prevention and control.
出处
《中国卫生检验杂志》
CAS
2016年第6期835-837,共3页
Chinese Journal of Health Laboratory Technology
关键词
呼吸道病毒
聚合酶链式反应
病原谱
Respiratory viruses
Polymerase chain reaction
Pathogen spectrum
