摘要
[目的]从海洋中分离并筛选具有杀松材线虫活性的细菌。[方法]采用稀释涂布平板法对采自青岛近海海域的样品进行细菌分离,采用浸渍法测定菌株的杀线虫活性,并对筛选菌株的形态学、生理生化特性和16S r DNA序列进行测定,通过单因素试验筛选产生杀线虫活性物质的最适培养条件。[结果]共分离到36株海洋细菌,根据杀线虫活性筛选出1株具有高杀线虫活性的细菌HT-11。该菌株分离自牡蛎,其培养滤液处理松材线虫24 h的校正死亡率高达92.41%。根据形态学、生理生化特性和16S r DNA序列测定及其系统发育分析结果,将菌株HT-11鉴定为考克氏菌属(Kocuria)。单因素试验结果表明,该菌株在营养肉汤(NB)培养基中产生杀线虫活性物质的最适培养条件为:海水浓度100%,初始p H 7.0,培养温度25℃,培养时间3 d。[结论]该研究可为海洋微生物资源的开发及其杀松材线虫天然活性物质的利用提供理论依据。
[Objective] To isolate and screen the marine bacteria with nematicidal activity against Bursaphelenchus xylophilus from the sea.[Method]The marine bacterial strains were isolated from the samples collected from the subtidal zones near Qingdao coast using the dilution and streak plate method; nematicidal activity of strains were detected by immersion method. The morphology,physiological and biochemical characters,and 16 S r DNA sequence of the screened strains were also detected. The optimal cultivation conditions to produce nematicidal activity substances were detected by single factor test.[Result]A total of 36 marine bacterial strains were isolated from the samples. One of these strains HT-11 had high nematicidal activity,which was isolated from oyster. Its corrected mortality reached as high as 92. 41% after treated with the culture filtrate for 24 h. According to the results of morphology,physiological and biochemical characters,16 S r DNA sequence and phylogenetic analysis,strain HT-11 was classified into the genus of Kocuria. Results of single factor experiment indicated that the optimal cultivation conditions of HT-11 for the production of nematicidal substances were 100% seawater concentration,initial p H 7,25 ℃ cultivation temperature,and 3 d cultivation time.[Conclusion]This research provides theoretical foundation for the development of marine microorganism resources and the utilization of natural nematicidal substances.
出处
《安徽农业科学》
CAS
2016年第4期24-28,共5页
Journal of Anhui Agricultural Sciences
基金
青岛市科技计划项目(13-1-4-158-jch)
关键词
海洋细菌
杀线虫活性
松材线虫
鉴定
培养条件
Marine bacteria
Nematicidal activity
Bursaphelenchus xylophilus
Identification
Culture conditions
