摘要
NAC类家族的基因是植物特有的转录因子,并且它在植物的生长发育中发挥着重要的作用。本研究以结缕草为研究材料,对结缕草转录组数据库利用local blast的办法克隆出1个NAC类转录因子。为深入探讨和研究NAC类转录因子在植物逆境生理上的作用奠定基础。通过与水稻的OsNACs家族成员进行进化树分析,结合蛋白分析,QRT-PCR等的方式探索其生物学功能。实验结果表明,该基因长度为1496bp,包含1个完整开放阅读框(ORF),长度为1332bp,编码449个氨基酸,该蛋白含有1个NAM结构域。与水稻的NAC类转录因子家族的系统进化树对比发现,它与OsNAC036近缘性最高,因此将该基因命名为ZjNAC036。QRT-PCR结果显示,该基因在干旱情况下表达量呈上升趋势,并且干旱处理8h的时候,差异倍数最大,达到了50倍左右,而在盐胁迫、低温胁迫下没有规律性变化。这表明ZjNAC036在干旱逆境生理上可能有重要的作用,而在高盐及寒冷的逆境生理方面可能作用不大。
Proteins in the NAC family（including NAM,ATAF1/2,and CUC2proteins）are plant-specific transcription factors that play critical roles in plant development.To explore and exploit the excellent resistancegene resources of zoysia（Zoysia japonica）,agene encoding a NAC transcription factor（ZjNAC）was cloned by blasting the Zoysia transcriptome database.The biological function of the cloned ZjNACgene was explored by aphylogenetic analysis with OsNACs of rice（Oryza sativa）combined with protein and quantitative reverse transcriptase polymerase chain reaction（qRT-PCR）analyses.The bioinformatics analysis showed the cloned ZjNACgene was 1496 bp long,and contained a 1332 bp open reading frame（ORF）encoding 449 amino acids with a NAM domain.In a phylogenetic analysis,the cloned ZjNACgene showed the highest homology to OsNAC036 from rice,and so the cloned gene was designated as ZjNAC036.The qRT-PCR analysis showed that ZjNACtranscript levels increased under drought stress,reaching peak levels（approximately 50-fold that in the control）after 8hof drought stress.The transcript levels of ZjNACdid not show obvious variations under salt stress or cold stress.Together,these results show that the protein encoded by ZjNAC036 may play an important role in the drought stress response,rather than the salt or cold stress responses.
出处
《草业学报》
CSCD
北大核心
2016年第4期239-245,共7页
Acta Prataculturae Sinica
基金
中央高校基本科研业务费专项资金(BLX2012034)
国家高新技术研究发展计划项目(2013AA102607)资助
关键词
结缕草
NAC转录因子
进化树
表达分析
Zoysia japonica
NAC transcription factor
phylogenetic tree
expression analysis
