摘要
用紫外-可见吸收光谱法和荧光光谱法研究聚肌胞(PPA)和牛血清白蛋白(BSA)的相互作用及共存金属离子对两者结合作用的影响.结果表明:PPA对BSA的荧光有猝灭作用,其猝灭过程属于动态猝灭.通过计算得出PPA与BSA的结合常数Kb及结合位点数n.根据热力学参数确定了PPA和BSA之间的作用力类型主要表现为疏水作用力.PPA在BSA中的结合位点主要位于ⅡA中.Hill系数略小于1,表明PPA对BSA的结合为弱的负协同作用.此外还详细探讨了共存金属离子对PPA与BSA结合作用的影响.
The interaction between polyinosinic-polycytidylic acid（PPA）and bovine serum albumin（BSA）and the effects of coexistent metal ions on their binding were studied with ultraviolet-visible absorption spectrometry（UV-VIS）and fluorescent spectrometry.The results showed that the fluorescence of BSA was quenched by PPA,which was a dynamic quenching process.The binding constant Kb and the corresponding number of binding sites n were obtained by calculation.The thermodynamic parameters obtained from the measured data showed that the interaction of PPA and BSA was mainly driven by the hydrophoic force.The primary binding site for PPA was located at the sub-domain ⅡA of BSA.The values of Hill＇s coefficients were less than 1,which indicated that there was a negative cooperative effect.In addition,the effects of coexistent metal ions on the binding constant of PPA with BSA were also studied.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第3期114-118,共5页
Journal of Southwest University(Natural Science Edition)
基金
国家自然科学基金资助项目(21261019)
云南省教育厅科学研究基金项目(2015C090Y)
关键词
聚肌胞
相互作用
金属离子
polyinosinic-polycytidylic acid
interaction
metal ion
