摘要
目的通过检测不同剂量的直链烷基苯磺酸钠(LAS)对人张氏肝细胞(Chang liver cells)增殖、损伤、凋亡及坏死的影响,探讨LAS对人张氏肝细胞的毒性作用。方法体外培养人张氏肝细胞,将细胞分为对照组及LAS不同剂量组,分别为正常对照组(不含LAS的正常培养基培养细胞)、LAS低、中、高剂量组(37.5、75.0及150.0 mg/L)。各组细胞培养24 h后,倒置显微镜下观察细胞形态及结构;四甲基偶氮唑盐(MTT)法检测细胞生存率;检测丙二醛(MDA)、乳酸脱氢酶(LDH)、天门冬氨酸氨基转移酶(AST)水平以了解肝细胞损伤程度;Hoechst 33342及PI染色观察细胞凋亡及坏死情况。结果 LAS处理后细胞表面出现黑色颗粒,部分细胞变圆脱落并产生碎片,高剂量组出现细胞脱落、死亡。LAS各处理组细胞存活率明显低于对照组(P<0.01),MDA、LDH及AST水平也均明显高于对照组;细胞凋亡率与坏死率明显增加(P<0.01),且这些变化随LAS剂量增加更加明显(P<0.01)。结论 LAS可抑制人张氏肝细胞增殖,改变细胞形态,并导致细胞损伤、凋亡和坏死。
Objective To investigate the toxicity of linear alkylbenzene sulfonate（ LAS） on human Chang liver cells. Methods Chang liver cells were cultured in complete growth medium,RPMI 1640 supplemented with 10% fetal calf serum and divided into control group（ cultured with normal medium without LAS）,LAS low,medium,high groups（ 37. 5,75. 0 and 150. 0 mg/L）. After being incubated for 24 h,morphological changes were observed under inverted microscope. The proliferation rate was measured by MTT assay. The injury of cells was evaluated through levels of malondialdehyde（ MDA）,lactate dehydrogenase（ LDH） and aspartate amine transfer（ AST）. Apoptosis and necrosis of Chang liver cells were explored using Hoechst 33342 and PI staining. Results When Chang liver cells were treated with different concentrations of LAS,morphological changes of cells were shown that there were black grains in the surface,the number of cells decreased,cast-off cells and cell breakage increased and there were large numbers of necrotic cells in higher-dose groups. Compared with the control group, the cell proliferation was inhibited,the levels of MDA,LDH and GOT increased and the rates ofapoptosis and necrosis enhanced significantly because of the treatment of LAS. Conclusion LAS can injure Chang liver cells in a way,it can inhibit cell proliferation and induce cells apoptosis.
出处
《卫生研究》
CAS
CSCD
北大核心
2016年第2期263-267,共5页
Journal of Hygiene Research
基金
安徽省高等学校自然科学重点研究项目(No.KJ2014A159)
安徽省高等学校省级质量工程教学研究项目(No.2014jyxm210)
安徽省高等学校省级质量工程特色专业建设项目(No.2014tszy019)
