摘要
聚酰胺-胺树形大分子(PAMAM)具有很强的荧光特性,在分子识别领域有广泛的应用。为研究PAMAM与腺苷三磷酸(ATP)之间的相互识别作用,本文采用发散合成法合成了多功能化的树形大分子G1.0 PAMAM,用核磁、红外光谱等方法对其结构进行表征,利用其多作用位点的特征,使用荧光光谱滴定法分析其对三磷酸腺苷(ATP)的识别作用性能,结果表明G1.0 PAMAM树形大分子(配体L)与ATP发生识别作用最佳p H为4,识别猝灭过程属于静态猝灭过程。根据Stern-Volmer方程,配体L与ATP识别的最佳配位比为1:1,猝灭常数为1.9447×104L/mol。上述研究结果表明:PAMAM与ATP有较强的识别作用,此识别作用对深入探索其对ATP水解催化的影响提供一定的参考价值。
PAMAM has strong fluorescence character and has been widely applied in molecular recognition. In order to investigate the interaction between PAMAM and Adenosine Triphosphate（ATP）. In this paper, mult- functional dendrimer G1.0 PAMAM（L） as a ligand was synthesized by divergent method. The product was characterized by FR- IR,1H NMR and13 C NMR.Making use of its many active sites, we studied for the recognition between ligand and ATP by fluorescence titration method. The result showed that the optimal p H of recognition intention between G1.0 PAMAM（L） and ATP is 4. The recognition quenching is a static quenching process.According to the Stern- Volmer equation, the optimum molar ratio of L and ATP is calculated as 1：1. The quenching constant is 1.9447 ×104L/mol. This result shows that there is strong recognition interaction between PAMAM and ATP. This recognition can support a reference value to explore the influence of PAMAM on the ATP hydrolysis.
出处
《石河子大学学报（自然科学版）》
CAS
2016年第1期100-105,共6页
Journal of Shihezi University(Natural Science)
基金
石河子材料化工重点实验室开放基金(2015BTRC002)
关键词
树形大分子
ATP
分子识别
荧光光谱法
dendrimer
ATP
molecular recognition
fluorescent spectra method
