摘要
目的:建立RP-HPLC法同时测定木豆叶中球松素、木豆素C的方法。方法:采用DiKMA Diamonsil ODS C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈、0.02%磷酸溶液梯度洗脱;流速:1.0mL·min^(-1);检测波长:296 nm;柱温:40℃。结果:球松素、木豆素C在0.002~0.02 mg·mL^(-1)范围内线性关系良好,r=0.999 4(球松素);r=0.999 5(木豆素C)。球松素平均回收率为99.0%,RSD为1.29%;木豆素C平均回收率为98.7%,RSD为1.72%。结论:该方法简单、准确可行,结果稳定可靠,可为木豆叶的质量控制提供科学依据。
OBJECTIVE To establish a RP-HPLC method for determination of pinostrobin and longistyle C in Folium Cajani.METHODS DiKMA Diamonsil ODS C18column（4.6 mm×250 mm,5μm）was adopted.Mobile phase was acetonitrile（solvent A）-0.02% phosphoric acid（solvent B）solution in gradient elution mode,at a flow rate of 1.0mL·min^-1,detection wavelength at 296 nm and column temperature at 40℃.RESULTS Good linearity was shown in rang of 0.002-0.02 mg·mL^-1 for pinostrobin（r=0.999 4）and longistyle C（r=0.999 5）.Average recovery rates of pinostrobin and longistyle C were99.0%（RSD=1.29%）and 98.7%（RSD=1.72%）.CONCLUSION The method is simple,accurate and feasible.The results are stable and reliable,and can provide a scientific basis for quality control of Folium Cajani.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2016年第6期440-443,共4页
Chinese Journal of Hospital Pharmacy
基金
国家自然基金(编号:30701131)
中国中医科学院中药研究所新药联合创制项目(编号:2011xycz-04)
郑州市金水区重点攻关项目(编号:金科2014-33)
河南省中医药科研专项(编号:2015ZY02010)
