摘要
目的:探讨葛根解酒胶囊对急性酒精中毒小鼠的预防及治疗作用。方法:"跳楼试验":选昆明小鼠30只,随机分为三组,每组10只,对照组:给予50%酒精0.2 ml·10g-1灌胃;药物治疗组:给予浓度为12 mg·ml-1的葛根解酒胶囊溶液0.2ml·10g-1灌胃,1h后各只给予50%酒精0.2ml·10g-1灌胃;非药物治疗组:给予生理盐水0.2ml·10g-1灌胃,1h后各只给予50%酒精0.2ml·10g-1灌胃;10min后将小鼠放到单杆上,观察并记录小鼠在单杆上停留的时间。水迷宫试验:经过水迷宫训练的昆明小鼠30只,随机分为3组,对照组:50%的酒精0.2ml·10g-1灌胃,20 min之后投入水迷宫;药物治疗组:50%的酒精0.2ml·10g-1灌胃,10min后浓度为12mg·ml-1葛根解酒胶囊溶液0.2ml·10g-1腹腔注射,再过10min后把小鼠投入水迷宫;非药物治疗组:50%的酒精0.2ml·10g-1灌胃,10min后生理盐水0.2ml·10g-1,腹腔注射,再过10min后把小鼠投入水迷宫,记录小鼠投入水迷宫后能否找到并爬上平台的只数,并记录其平均游泳速度。结果:药物治疗组维持平衡时间(266.9±52.4s)明显长于非药物治疗组(173.5±74.1s)和对照(155.8±54.8s)(P<0.01);葛根解酒胶囊能够促进小鼠找到平台(P<0.05),药物治疗组小鼠的游泳速度(17.2±9.4cm·s-1)比非药物治疗组(8.02±4.8cm·s-1)及对照组(7.01±4.1cm·s-1)快(P<0.05)。结论:葛根解酒胶囊对急性酒精中毒有一定预防及治疗作用。
Objective：To study the prevention and treatment effects of puerariae anti-inebriation capsule on mice with acute alcohol intoxication.Methods：Jump experiment：Thirty Kunming mice were randomly divided into three groups（n=10）.Control group：each mouse was given 50% alcohol by intragastric administration（dosage,0.2 ml·10g^-1）.Drug-treatment group：each mouse was fed with the solution of puerariae anti-inebriation capsule（concentration,12mg·ml^-1,dosage,0.2ml·10g^-1）,then with 50% alcohol（dosage,0.2ml·10g^-1）after one hour by the way of intragastric administration.Non-drug treatment group：all mice were given normal saline（dosage,0.2ml·10g^-1）,one hour later,fed with 50%alcohol（dosage,0.2ml·10g^-1）through the way as before.When finishing conductions of each group respectively,mice were put on a single pole after ten minutes,to observe the length of time the mice stay on the pole.Morris water maze experiment：Another thirty Kunming mice which have been trained with Morris water maze experiment were randomly divided into three groups（n=10）.Control group：50% alcohol（dosage,0.2ml·10g^-1）was given to each mouse in this group by intragastric administration.Then the mice were launched to Morris water maze after twenty minutes.Drug-treatment group：first,all mice which were divided into this group were received 50% alcohol（dosage,0.2ml·10g^-1）by intragastric administration.Second,they were treated with the solution of puerariae anti-inebriation capsule（concentration,12mg·ml^-1,dosage,0.2ml·10g^-1）by intraperitoneal injection ten minutes later.Third,they were launched to Morris water maze after another ten minutes.Non-drug treatment group：In this group,the mice were treated as the same way as drug-treatment group excepting the second step.At the second step,the mice of non-drug treatment group were only given normal saline（dosage,0.2ml·10g^-1）by intraperitoneal injection ten minutes after the first step.In this experiment,we need to observe whether the mice
出处
《四川生理科学杂志》
2016年第1期15-18,共4页
Sichuan Journal of Physiological Sciences
关键词
葛根解酒胶囊
急性酒精
预防及治疗作用
Puerariae anti-inebriation
Acute alcoholism
Prevention therapeutic action
