摘要
目的探讨磷酸二酯酶抑制剂米力农对家兔顿抑心肌的治疗效果。方法将新西兰家兔40只通过简单随机抽样为对照组、完全缺血组、心肌顿抑组和米力农组,各10只。对照组:不阻断家兔冠状动脉;缺血组:完全阻断家兔冠状动脉并不再开放;心肌顿抑组:阻断家兔冠状动脉30 min,开放60 min;米力农组:通过结扎冠状动脉左旋支30 min,开放60 min建立心肌顿抑模型。通过检测不同时间点(实验30 min,60 min,90 min)的肌钙蛋白指标变化及病理改变了解米力农对心肌细胞的影响,通过细胞凋亡的流式实验检测家兔心肌凋亡情况。结果对照组cTn I在整个实验过程中无明显变化[(45±7),(46±10),(45±7)mg/L]。缺血组[(279±5),(361±23),(482±34)mg/L]及心肌顿抑组[(281±3),(377±20),(494±26)mg/L]较对照组c Tn I显著增高,差异有统计学意义(P<0.05)。米力农组:实验60 min、90 min c Tn I较实验30 min时逐渐降低[(88±6)mg/L、(50±6)mg/L比(119±6)mg/L](P<0.05),心肌顿抑组和缺血组凋亡率和坏死率显著高于对照组[(36.2±8.1)%,(27.6±3.7)%比(21.0±2.4)%;(10.4±3.1)%,(17.6±3.8)%比(3.0±0.5)%](P<0.05)。心肌顿抑组凋亡率高于缺血组(P<0.05),而坏死率显著低于缺血组(P<0.05)。米力农组心肌细胞坏死率[(4.6±0.9)%]和凋亡率[(23.0±3.7)%]均显著低于心肌顿抑组(P<0.05),心肌细胞坏死率低于缺血组(P<0.05)。结论米力农能够减少家兔缺血/再灌注损伤所诱导的心肌细胞凋亡。
Objective To investigate the effects of milrinone on myocardial stunning of rabbits.Methods Forty New Zealand rabbits were divided into 4 groups randomly： control group,ischemia group,myocardial stunning group,milrinone group,10 rabbits each. In the control group： coronary artery not blocked in rabbits; ischemia group： rabbit coronary artery was completely blocked and was no longer open; myocardial stunning group： rabbits coronary artery blocked 30 min,open 60 min; milrinone group： ligation of left coronary artery for 30 min,open 60 min to establish myocardial stunning model. c Tn I at different time points（ experiment 30,60,90 min） were monitored to understand heart function changes and milrinone effect on cardiocytes. Apoptotic cardiomyocyte was detected flow cytometry of apoptosis method. Results The c Tn I of the control group had no obvious change in the process of the whole experiment [（ 45 ± 7）,（ 46 ± 10）,（ 45 ± 7） mg/L]. The c Tn I of the ischemia group [（ 279 ± 5）,（ 361 ± 23）,（ 482 ± 34） mg/L]and the myocardial stunning group [（ 281 ± 3）,（ 77 ± 20）,（ 494 ± 26） mg / L]was significantly higher than the control group,the differences were statistically significant（ P 〈0. 05）. Milrinone group： c Tn I at experiment 60 min,and 90 min was lower than at 30 min[（ 88 ± 6） mg / L,（ 50 ± 6） mg / Lvs（ 119 ± 6） mg / L,P 〈0. 05]. Myocardial apoptosis rate and myocardial necrosis rate of myocardial stunning group and ischemia group were significantly higher than control group[（ 36. 2 ± 8. 1） %,（ 27. 6 ± 3. 7） % vs（ 21. 0 ± 2. 4） %;（ 10. 4 ±3. 1） %,（ 17. 6 ± 3. 8） % vs（ 3. 0 ± 0. 5） % ]（ P 〈0. 05）. Myocardial apoptosis rate of myocardial stunning group was significantly higher than the ischemia group（ P 〈0. 05）,and myocardial necrosis rate was significantly lower than the ischemia group（ P 〈0. 05）. The apoptosis rate[（ 4. 6 ± 0. 9） %] and the necrosis rate[（ 23. 0 ± 3. 7） %]of milri
出处
《医学综述》
2016年第6期1186-1189,F0003,共5页
Medical Recapitulate
关键词
心肌顿抑
米力农
心肌细胞
肌钙蛋白
凋亡
Myocardial stunning
Milrinone
Cardiomyocyte
Cardiac troponin
Apoptosis
