摘要
目的观察针刺联合丰富康复训练对脑缺血损伤大鼠神经功能及损伤侧皮质NGF、BDNF表达的影响。方法线栓法制作急性局灶性脑缺血损伤动物模型,动物随机分为假手术组、模型组、针刺组、丰富康复训练组及针康组(针刺+丰富康复训练)5组,每组进行神经缺损体征评分和感觉、运动能力评分,应用免疫组织化学技术进行NGF、BDNF显色,分析比较各组大鼠脑组织中阳性细胞的表达情况。结果治疗前行为学功能评分各组无明显差异(P>0.05),行为学功能评分针康组与模型组比较有极显著性差异(P<0.01),与针刺组及丰富康复训练组比较有显著性差异(P<0.05);与假手术组比较,模型组大鼠皮质NGF、BDNF的表达显著升高(P<0.01);与模型组比较,治疗组大鼠皮质NGF、BDNF的表达均显著升高(P<0.01);与针刺组及丰富康复训练组相比,针康组效果显著(P<0.05)。结论针刺联合丰富康复训练对脑缺血损伤大鼠神经功能有保护和修复作用,疗效优于针刺法及丰富康复训练法,其效应可能上调大鼠NGF、BDNF蛋白的表达有关。
Objective To observe the effects of acupuncture combined with enriched rehabilitation training on neural function and expression of cortical nerve growth factor（NGF）and brain derived neurophic factor（BDNF）in rats after focal cerebral ischemia injury. Methods Rat models of local cerebral ischemia were established by thread-occluding the middle cerebral artery（MCAO）. Rats were randomly divided into sham operated,model,acupuncture,enriched rehabilitation training and acupuncture combined enriched rehabilitation training groups. Marks of neural function defect、sensory and motor ability were noted down separately. The immunohistochemistry staining was applied to observe the expression of NGF and BDNF cells in rats. The expression of NGF and BDNF were analyzed after cerebral ischemia among the above groups. Results Behavior medicine scopes of rats in every group had no obvious difference before therapy（P〉0.05）. The improvement of acupuncture combined enriched rehabilitation training group was significant（P〈0.01）,while the difference in comparison with acupuncture group and enriched rehabilitation training group were remarkable（P〈0.05）. Compared with the sham operation group,cortical NGF and BDNF expressions increased significantly in the model group（P 〈0.01）. Compared with the model group,cortical NGF and BDNF expressions increased significantly in the treatment groups（P 〈0.01）. Compared with the acupuncture group and enriched rehabilitation training group, the effect of acupuncture combined enriched rehabilitation training group was marked in the treatment group（P〈0.05）. Conclusion The therapy of acupuncture combined enriched rehabilitation training can promote recovery of behavior in rats after focal cerebral ischemia and it was better the therapy of simple enriched rehabilitation training and acupuncture. the mechanism may be related to increasing the expression of NGF and BDNF in cortex.
出处
《云南中医学院学报》
2016年第1期1-5,共5页
Journal of Yunnan University of Traditional Chinese Medicine
基金
国家自然科学基金(30873293
81373711
81102547)
关键词
针刺
丰富康复训练
脑缺血损伤
脑源性神经营养因子
神经生长因子
acupuncture
enriched rehabilitation training
cerebral ischemia injury
brain derived neurophic factor
nerve growth factor
