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杜仲叶片不同发育时期数字基因表达谱分析 被引量:1

Analysis of Digital Gene Expression about Eucommia ulmoides Oliver Leaves at Different Developmental Stages
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摘要 为在转录水平解析杜仲叶片发育过程中的基因表达模式,该研究通过数字基因表达谱技术对‘华仲6号’杜仲叶片从展叶(4月)到落叶(10月)不同发育时期的基因表达水平进行比较分析,共获得差异基因3 002个,其中1 764个表达量上调,1 238个下调,这些差异表达基因主要行使催化、氧化还原等功能,参与代谢过程和生物过程等;进一步Pathway富集分析发现,苯丙素类生物合成途径相关基因被富集,其中调控绿原酸合成的6个基因差异表达显著;对这些基因表达模式进行分析显示,4月中旬和9月中旬基因的表达量较高,暗示这两个时期对于绿原酸的合成具有重要作用。荧光定量RT-PCR检测6个绿原酸合成相关基因和12个随机选择的差异表达基因,验证结果显示表达谱测序结果可靠。该研究结果为揭示杜仲叶片在不同发育时期的分子调控机制奠定了基础,并为深入解析杜仲绿原酸合成机理,进而通过分子育种手段提高杜仲绿原酸含量提供新的路径。 To clarify the gene expression patterns of Eucommia ulmoides Oliver leaves in the process of development at the transcription level,this study compared and analyzed the gene expression level of‘Huazhong 6’leaves in different development periods from expanding(April)to falling(October)by digital gene expression profile technology.A total of 3 002 different expression genes were obtained,in which1 764up-regulated expression and 1 238down-regulated expression.These mainly function referred to catalytic activity or oxidoreductase activity,and involved in metabolic process and biological process.Through further pathway enrichment analysis,we found that several related genes of phenylpropanoid biosynthesis pathway were enriched,in which six significantly different expression genes were related to chlorogenic acid synthesis.The gene expression patterns showed that these genes are highly expressed in mid-April and mid-Septemeber,which suggested that these two periods play an important role for chlorogenic acid synthesis.By real-time fluorescent quantitative PCR,6chlorogenic acid synthesis genes and 12 randomly different expression genes were detected,and the results verified the reliability of digital gene expression profile.This study lays a solid foundation for revealing the molecular regulation mechanism of E.ulmoides Oliver leaves in different development periods,and provides the reference for further clarifying the synthesis mechanism of E.ulmoides Oliver chlorogenic acid and then improve E.ulmoides Oliver chlorogenic acid content by means of molecular breeding.
出处 《西北植物学报》 CAS CSCD 北大核心 2016年第2期257-265,共9页 Acta Botanica Boreali-Occidentalia Sinica
基金 国家十二五科技支撑计划(2012BAD21B0502) 国家公益性行业科研专项(201004029)
关键词 杜仲叶片 数字基因表达谱 差异基因 绿原酸 Eucommia ulmoides Oliver leaf digital gene expression profile different expression gene chlorogenic acid
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